TY - JOUR
T1 - Optimization of Light and Nutrients Supply to Stabilize Long-Term Industrial Cultivation of Metabolically Engineered Cyanobacteria
T2 - A Model-Based Analysis
AU - Battaglino, Beatrice
AU - Arduino, Alessandro
AU - Pagliano, Cristina
AU - Sforza, Eleonora
AU - Bertucco, Alberto
N1 - Publisher Copyright:
© 2021 American Chemical Society.
PY - 2021/7/28
Y1 - 2021/7/28
N2 - Metabolically engineered cyanobacteria are promising photosynthetic cell factories to produce valuable compounds in view of a bio-based industry. However, when the producer population is affected by a production burden, it usually experiences genetic instability leading to cells that lose the production capability, here defined as retro-mutants, which in the long-term take over the culture. Here we show that by exploitation of differences in nutrient and light use between these two phenotypes, in a continuous culture the operative conditions can be set to specifically select the producers. A mathematical model-based analysis used to investigate the effect of kinetic parameters shows that in specific combinations of their values, a continuous stirred-tank reactor (CSTR) can be operated to favor the producer's growth. The feasibility of the approach proposed is discussed in the context of literature data. On the basis of overall mass and energy balance analysis, a new approach to stabilize the producer phenotype in long-term industrial cultivation is proposed.
AB - Metabolically engineered cyanobacteria are promising photosynthetic cell factories to produce valuable compounds in view of a bio-based industry. However, when the producer population is affected by a production burden, it usually experiences genetic instability leading to cells that lose the production capability, here defined as retro-mutants, which in the long-term take over the culture. Here we show that by exploitation of differences in nutrient and light use between these two phenotypes, in a continuous culture the operative conditions can be set to specifically select the producers. A mathematical model-based analysis used to investigate the effect of kinetic parameters shows that in specific combinations of their values, a continuous stirred-tank reactor (CSTR) can be operated to favor the producer's growth. The feasibility of the approach proposed is discussed in the context of literature data. On the basis of overall mass and energy balance analysis, a new approach to stabilize the producer phenotype in long-term industrial cultivation is proposed.
UR - http://www.scopus.com/inward/record.url?scp=85101414782&partnerID=8YFLogxK
U2 - 10.1021/acs.iecr.0c04887
DO - 10.1021/acs.iecr.0c04887
M3 - Article
SN - 0888-5885
VL - 60
SP - 10455
EP - 10465
JO - Industrial & Engineering Chemistry Research
JF - Industrial & Engineering Chemistry Research
IS - 29
ER -