On the role of thiol groups in the inhibition of liver microsomal ca2+ sequestration by toxic agents

Hjördis Thor, Pia Hartzell, Sten Åke Svensson, Sten Orrenius, Francesca Mirabelli, Vito Marinoni, Giorgio Bellomo

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

ATP-dependent Ca2+ sequestration by rat liver microsomes was assayed using three different methods, and characterized with regard to the effect of various inhibitors. When glucose and hexokinase were added in combination to deplete ATP in the incubation, Ca2+ uptake was followed by rapid release of Ca2+ from the microsomes. Ca2+ sequestration was inhibited by reagents that cause alkylation (e.g. p-chloromercuribenzoate) or oxidation (e.g. diamide) of protein sulfhydryl groups. Moreover, pretreatment of the microsomes with cystamine, which causes formation of mixed disulfides with protein thiols, also resulted in the inhibition of Ca2+ sequestration. It is concluded that microsomal Ca2+ sequestration is critically dependent on protsin sulfhydryl groups, and that modification of protein thiols may be an important mechanism for the inhibition of microsomal Ca12+ sequestration by a variety of toxic agents.

Lingua originaleInglese
pagine (da-a)3717-3723
Numero di pagine7
RivistaBiochemical Pharmacology
Volume34
Numero di pubblicazione20
DOI
Stato di pubblicazionePubblicato - 15 ott 1985
Pubblicato esternamente

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