TY - JOUR
T1 - Nuclear factor kB is activated by arachidonic acid but not by eicosapentaenoic acid
AU - Camandola, Simonetta
AU - Leonarduzzi, Gabriella
AU - Musso, Tiziana
AU - Varesio, Luigi
AU - Carini, Rita
AU - Scavazza, Antonella
AU - Chiarpotto, Elena
AU - Baeuerle, Patrick A.
AU - Poli, Giuseppe
N1 - Funding Information:
This work was supported by grants from Italian Ministry for University, Scientific and Technological Research, Italian Ministry of Health VI AIDS Project, National Research Council Targeted Project ‘‘ACRO,’’ and Italian Association for Cancer Research.
PY - 1996/12/13
Y1 - 1996/12/13
N2 - The Ω-6 arachidonic acid supplementation of the human promonocytic cell line U937 strongly stimulates the nuclear translocation of the transcription factor NF-kB. Inhibitors of arachidonate oxidative metabolism prevent NF-kB activation, indirectly indicating a role for prostaglandin and leukotriene metabolites in the genesis of this phenomenon. Of note, Ω-3 eicosapentaenoic acid does not exert any effect on NF-kB DNA binding. In subsequent experiments, prostaglandin E2 consistently showed the ability to activate NF-kB in U937 promonocytic cells, as well as in J774 macrophages. NF-kB activation by arachidonate, together with the lack of effect by eicosapentaenoic acid, suggests a way to modulate the expression of certain genes by means of a suitable dietary n-6/n-3 fatty acid ratio.
AB - The Ω-6 arachidonic acid supplementation of the human promonocytic cell line U937 strongly stimulates the nuclear translocation of the transcription factor NF-kB. Inhibitors of arachidonate oxidative metabolism prevent NF-kB activation, indirectly indicating a role for prostaglandin and leukotriene metabolites in the genesis of this phenomenon. Of note, Ω-3 eicosapentaenoic acid does not exert any effect on NF-kB DNA binding. In subsequent experiments, prostaglandin E2 consistently showed the ability to activate NF-kB in U937 promonocytic cells, as well as in J774 macrophages. NF-kB activation by arachidonate, together with the lack of effect by eicosapentaenoic acid, suggests a way to modulate the expression of certain genes by means of a suitable dietary n-6/n-3 fatty acid ratio.
UR - https://www.scopus.com/pages/publications/0030582692
U2 - 10.1006/bbrc.1996.1857
DO - 10.1006/bbrc.1996.1857
M3 - Article
SN - 0006-291X
VL - 229
SP - 643
EP - 647
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -