TY - JOUR
T1 - Novel polyurethane-based thermosensitive hydrogels as drug release and tissue engineering platforms
T2 - Design and in vitro characterization
AU - Boffito, Monica
AU - Gioffredi, Emilia
AU - Chiono, Valeria
AU - Calzone, Stefano
AU - Ranzato, Elia
AU - Martinotti, Simona
AU - Ciardelli, Gianluca
N1 - Publisher Copyright:
© 2016 Society of Chemical Industry.
PY - 2016/7/1
Y1 - 2016/7/1
N2 - Poloxamer P407 (P407) is a Food and Drug Administration approved triblock copolymer; its hydrogels show fast dissolution in aqueous environment and weak mechanical strength, limiting their in vivo application. In this work, an amphiphilic poly(ether urethane) (NHP407) was synthesized from P407, an aliphatic diisocyanate (1,6-hexanediisocyanate) and an amino acid derived diol (N-Boc serinol). NHP407 solutions in water-based media were able to form biocompatible injectable thermosensitive hydrogels with a lower critical gelation temperature behavior, having lower critical gelation concentration (6% w/v versus 18% w/v), superior gel strength (G′ at 37°C about 40000Pa versus 10000Pa), faster gelation kinetics (<5min versus 15-30min) and higher stability in physiological conditions (28days versus 5 days) compared to P407 hydrogels. Gel strength and PBS absorption at 37°C increased whereas dissolution rate (in phosphate-buffered saline (PBS) at 37°C) and permeability to nutrients (studied using fluorescein isothiocyanate-dextran model molecule) decreased as a function of NHP407 hydrogel concentration from 10% to 20% w/v. By varying the concentration, NHP407 hydrogels were thus prepared with different properties which could suit specific applications, such as in situ drug/cell delivery or bioprinting of scaffolds. Moreover, deprotected amino groups in NHP407 could be exploited for the grafting of bioactive molecules obtaining biomimetic hydrogels.
AB - Poloxamer P407 (P407) is a Food and Drug Administration approved triblock copolymer; its hydrogels show fast dissolution in aqueous environment and weak mechanical strength, limiting their in vivo application. In this work, an amphiphilic poly(ether urethane) (NHP407) was synthesized from P407, an aliphatic diisocyanate (1,6-hexanediisocyanate) and an amino acid derived diol (N-Boc serinol). NHP407 solutions in water-based media were able to form biocompatible injectable thermosensitive hydrogels with a lower critical gelation temperature behavior, having lower critical gelation concentration (6% w/v versus 18% w/v), superior gel strength (G′ at 37°C about 40000Pa versus 10000Pa), faster gelation kinetics (<5min versus 15-30min) and higher stability in physiological conditions (28days versus 5 days) compared to P407 hydrogels. Gel strength and PBS absorption at 37°C increased whereas dissolution rate (in phosphate-buffered saline (PBS) at 37°C) and permeability to nutrients (studied using fluorescein isothiocyanate-dextran model molecule) decreased as a function of NHP407 hydrogel concentration from 10% to 20% w/v. By varying the concentration, NHP407 hydrogels were thus prepared with different properties which could suit specific applications, such as in situ drug/cell delivery or bioprinting of scaffolds. Moreover, deprotected amino groups in NHP407 could be exploited for the grafting of bioactive molecules obtaining biomimetic hydrogels.
KW - Bioprinting
KW - Drug release
KW - Poloxamer
KW - Polyurethane hydrogels
KW - Thermosensitive hydrogels
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=84959533057&partnerID=8YFLogxK
U2 - 10.1002/pi.5080
DO - 10.1002/pi.5080
M3 - Article
SN - 0959-8103
VL - 65
SP - 756
EP - 769
JO - Polymer International
JF - Polymer International
IS - 7
ER -