TY - JOUR
T1 - Novel MRI and fluorescent probes responsive to the Factor XIII transglutaminase activity
AU - Tei, Lorenzo
AU - Mazooz, Galit
AU - Shellef, Yael
AU - Avni, Reut
AU - Vandoorne, Katrien
AU - Barge, Alessandro
AU - Kalchenko, Vyacheslav
AU - Dewhirst, Mark W.
AU - Chaabane, Linda
AU - Miragoli, Luigi
AU - Longo, Dario
AU - Neeman, Michal
AU - Aime, Silvio
PY - 2010/7
Y1 - 2010/7
N2 - Transglutaminases, including factor XIII and tissue transglutaminase, participate in multiple extracellular processes associated with remodeling of the extracellular matrix during wound repair, blood clotting, tumor progression and fibrosis of ischemic injuries. The aim of this work was to evaluate a novel substrate analog for transglutaminase optimized by molecular modeling calculations (DCCP16), which can serve for molecular imaging of transglutaminase activity by magnetic resonance imaging and by near-infrared imaging. Experimental data showed covalent binding of Gd-DCCP16 and DCCP16-IRIS Blue to human clots, to basement membrane components and to casein in purified systems as well as in three-dimensional multicellular spheroids. In vivo, DCCP16 showed enhancement with a prolonged retention in clots and tumors, demonstrating the ability to detect both factor XIII and tissue transglutaminase mediated covalent binding of the contrast material.
AB - Transglutaminases, including factor XIII and tissue transglutaminase, participate in multiple extracellular processes associated with remodeling of the extracellular matrix during wound repair, blood clotting, tumor progression and fibrosis of ischemic injuries. The aim of this work was to evaluate a novel substrate analog for transglutaminase optimized by molecular modeling calculations (DCCP16), which can serve for molecular imaging of transglutaminase activity by magnetic resonance imaging and by near-infrared imaging. Experimental data showed covalent binding of Gd-DCCP16 and DCCP16-IRIS Blue to human clots, to basement membrane components and to casein in purified systems as well as in three-dimensional multicellular spheroids. In vivo, DCCP16 showed enhancement with a prolonged retention in clots and tumors, demonstrating the ability to detect both factor XIII and tissue transglutaminase mediated covalent binding of the contrast material.
KW - Blood clotting
KW - Magnetic resonance imaging
KW - Molecular imaging
KW - Near infrared imaging
KW - Responsive contract agents
KW - Transglutaminase activity
KW - Tumor imaging
UR - http://www.scopus.com/inward/record.url?scp=77956150271&partnerID=8YFLogxK
U2 - 10.1002/cmmi.392
DO - 10.1002/cmmi.392
M3 - Article
SN - 1555-4309
VL - 5
SP - 213
EP - 222
JO - Contrast Media and Molecular Imaging
JF - Contrast Media and Molecular Imaging
IS - 4
ER -