@article{de8351c1b239408c93cc7edfc24c97bf,
title = "NMR Spectroscopic Assignment of Backbone and Side-Chain Protons in Fully Protonated Proteins: Microcrystals, Sedimented Assemblies, and Amyloid Fibrils",
abstract = "We demonstrate sensitive detection of alpha protons of fully protonated proteins by solid-state NMR spectroscopy with 100–111 kHz magic-angle spinning (MAS). The excellent resolution in the Cα-Hα plane is demonstrated for 5 proteins, including microcrystals, a sedimented complex, a capsid and amyloid fibrils. A set of 3D spectra based on a Cα–Hα detection block was developed and applied for the sequence-specific backbone and aliphatic side-chain resonance assignment using only 500 μg of sample. These developments accelerate structural studies of biomolecular assemblies available in submilligram quantities without the need of protein deuteration.",
keywords = "magic-angle spinning, proton detection, resonance assignment, solid-state NMR spectroscopy",
author = "Jan Stanek and Andreas, \{Loren B.\} and Kristaps Jaudzems and Diane Cala and Daniela Lalli and Andrea Bertarello and Tobias Schubeis and Inara Akopjana and Svetlana Kotelovica and Kaspars Tars and Andrea Pica and Serena Leone and Delia Picone and Xu, \{Zhi Qiang\} and Dixon, \{Nicholas E.\} and Denis Martinez and M{\'e}lanie Berbon and \{El Mammeri\}, Nadia and Abdelmajid Noubhani and Sven Saupe and Birgit Habenstein and Antoine Loquet and Guido Pintacuda",
note = "Publisher Copyright: {\textcopyright} 2016 Wiley-VCH Verlag GmbH \& Co. KGaA, Weinheim",
year = "2016",
month = dec,
day = "12",
doi = "10.1002/anie.201607084",
language = "English",
volume = "55",
pages = "15504--15509",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley and Sons Ltd",
number = "50",
}