Nicotine stimulation increases proliferation and matrix metalloproteinases-2 and -28 expression in human dental pulp cells

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

Aims: Dental pulp is the specialized tissue responsible for maintaining tooth viability. When tooth mineralized matrix is damaged, pulp is exposed to a plethora of environmental stimuli. In particular, in smokers, pulp become exposed to very high concentrations of nicotine. The aim of this study was to investigate the effect of direct nicotine stimulation on human dental pulp cell proliferation. Moreover, as it is known that nicotine could upregulate the expression of matrix metalloproteinases (MMPs), enzymes involved in pulpal inflammation, the effects of nicotine stimulation on MMP-2 and MMP-28 gene expression have also been investigated. Main methods: Human dental pulp cells were extracted from impacted third molars obtained from healthy patients undergoing routine orthodontic treatments. Such cells were treated with growing concentrations of nicotine in the presence or absence of a nicotine antagonist (hexamethonium chloride) or of a MEK signaling inhibitor (PD98059). Cell proliferation was evaluated by cell counting,while nicotine effects onMMP expression were evaluated by PCR. Key findings: The data obtained indicate that nicotine is able to increase human dental pulp cell proliferation by acting through nicotinic cholinergic receptors and downstream MAPK signaling pathway. Moreover, it is also able to increase both MMP-2 and MMP-28 gene expression. Significance: In summary these results highlight that direct exposure of human dental pulp cells to nicotine results in an inflammatory response, that could have a role in pulpal inflammation onset, a pathological condition that, when ignored, could eventually spread to the surrounding alveolar bone and progress to pulp necrosis.

Lingua originaleInglese
pagine (da-a)49-54
Numero di pagine6
RivistaLife Sciences
Volume135
DOI
Stato di pubblicazionePubblicato - 22 giu 2015

Fingerprint

Entra nei temi di ricerca di 'Nicotine stimulation increases proliferation and matrix metalloproteinases-2 and -28 expression in human dental pulp cells'. Insieme formano una fingerprint unica.

Cita questo