TY - JOUR
T1 - Nanostructured scaffold with biomimetic and antibacterial properties for wound healing produced by ‘green electrospinning’
AU - Tonda-Turo, Chiara
AU - Ruini, Francesca
AU - Ceresa, Chiara
AU - Gentile, Piergiorgio
AU - Varela, Patrícia
AU - Ferreira, Ana M.
AU - Fracchia, Letizia
AU - Ciardelli, Gianluca
N1 - Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Hypothesis: Wound healing is a complex process that often requires treatment with antibacterial agents to avoid infection, which affects the optimal tissue regeneration process. Ideal scaffolds for wound healing treatment should combine biomimetic features to ensure the tissue growth on properly designed extracellular matrix (ECM)-like scaffolds and antibacterial properties in order to avoid bacterial colonization. Experiments: In this work, gelatin cross-linked nanofibers (GL-nanofibres), with diameters ranging from 200 to 300 nm, were prepared via a “green electrospinning technique” to mimic the structure and composition of the extracellular matrix (ECM), and promote the normal skin wound healing process. Nanofibres were doped with two antibacterial agents (gentamicin sulphate or silver nanoparticles) to achieve an antibacterial effect against Gram + and Gram- bacteria. Findings: The ECM-mimicking structure of GL-nanofibres was not affected by the presence of the antibacterial agents, which were homogeneously distributed within the mats as shown by SEM and EDS. The antibacterial properties of the developed matrices were confirmed using 4 strains (S. aureus, S. epidermidis, P. aeruginosa and E. coli) while the biocompatibility of the developed substrates and their ability to induce cell growth was assessed using Neonatal Normal Human Dermal Fibroblasts (NHDF-Neo).
AB - Hypothesis: Wound healing is a complex process that often requires treatment with antibacterial agents to avoid infection, which affects the optimal tissue regeneration process. Ideal scaffolds for wound healing treatment should combine biomimetic features to ensure the tissue growth on properly designed extracellular matrix (ECM)-like scaffolds and antibacterial properties in order to avoid bacterial colonization. Experiments: In this work, gelatin cross-linked nanofibers (GL-nanofibres), with diameters ranging from 200 to 300 nm, were prepared via a “green electrospinning technique” to mimic the structure and composition of the extracellular matrix (ECM), and promote the normal skin wound healing process. Nanofibres were doped with two antibacterial agents (gentamicin sulphate or silver nanoparticles) to achieve an antibacterial effect against Gram + and Gram- bacteria. Findings: The ECM-mimicking structure of GL-nanofibres was not affected by the presence of the antibacterial agents, which were homogeneously distributed within the mats as shown by SEM and EDS. The antibacterial properties of the developed matrices were confirmed using 4 strains (S. aureus, S. epidermidis, P. aeruginosa and E. coli) while the biocompatibility of the developed substrates and their ability to induce cell growth was assessed using Neonatal Normal Human Dermal Fibroblasts (NHDF-Neo).
KW - Antibacterial nanofibers
KW - ECM-like scaffolds
KW - Electrospinning
KW - Gelatin
KW - Gentamicin
KW - Silver nanoparticles
KW - Wound healing
UR - http://www.scopus.com/inward/record.url?scp=85052449432&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2018.08.039
DO - 10.1016/j.colsurfb.2018.08.039
M3 - Article
SN - 0927-7765
VL - 172
SP - 233
EP - 243
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -