Multiple myeloma shows no intra-disease clustering of immunoglobulin heavy chain genes

S Ferrero; Daniela CAPELLO; M Svaldi; M Boi; D Gatti; D Drandi; Davide ROSSI; S Barbiero; B Mantoan; E Mantella; M Zanni; P Ghione; A Larocca; R Passera; F Bertoni; V Gattei; F Forconi; L Laurenti; Poeta G Del; R Marasca; S Cortelazzo; Gianluca GAIDANO; A Palumbo; M Boccadoro; Marco LADETTO

doi:10.3324/haematol.2011.052852

Multiple myeloma shows no intra-disease clustering of immunoglobulin heavy chain genes

S Ferrero, Daniela CAPELLO, M Svaldi, M Boi, D Gatti, D Drandi, Davide ROSSI, S Barbiero, B Mantoan, E Mantella, M Zanni, P Ghione, A Larocca, R Passera, F Bertoni, V Gattei, F Forconi, L Laurenti, Poeta G Del, R MarascaS Cortelazzo, Gianluca GAIDANO, A Palumbo, M Boccadoro, Marco LADETTO

Dipartimento di Medicina Traslazionale

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

BACKGROUND: Characterization of the immunoglobulin gene repertoire has improved our understanding of the immunopathogenesis of lymphoid tumors. Early B-lymphocyte precursors of multiple myeloma are known to exist and might be susceptible to antigenic drive. DESIGN AND METHODS: To verify this hypothesis, we collected a database of 345 fully readable multiple myeloma immunoglobulin sequences. We characterized the immunoglobulin repertoire, analyzed the somatic hypermutation load, and investigated for stereotyped receptor clusters. RESULTS: Compared to the normal immunoglobulin repertoire, multiple myeloma displayed only modest differences involving only a few genes, showing that the myeloma immunoglobulin repertoire is the least skewed among mature B-cell tumors. Median somatic hypermutation load was 7.8%; median length of complementarity determining-region 3 was 15.5 amino acids. Clustering analysis showed the absence of myeloma specific clusters and no similarity with published chronic lymphocytic leukemia or lymphoma subsets. CONCLUSIONS: Analysis of multiple myeloma immunoglobulin repertoire does not support a pathogenetic role for antigen selection in this tumor.

Lingua originale	Inglese
pagine (da-a)	849-853
Numero di pagine	5
Rivista	Haematologica
Volume	97
Numero di pubblicazione	6
DOI	https://doi.org/10.3324/haematol.2011.052852
Stato di pubblicazione	Pubblicato - 2012

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10.3324/haematol.2011.052852

http://hdl.handle.net/11579/31441

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Ferrero, S., CAPELLO, D., Svaldi, M., Boi, M., Gatti, D., Drandi, D., ROSSI, D., Barbiero, S., Mantoan, B., Mantella, E., Zanni, M., Ghione, P., Larocca, A., Passera, R., Bertoni, F., Gattei, V., Forconi, F., Laurenti, L., Del, P. G., ... LADETTO, M. (2012). Multiple myeloma shows no intra-disease clustering of immunoglobulin heavy chain genes. Haematologica, 97(6), 849-853. https://doi.org/10.3324/haematol.2011.052852

@article{b04bfc87de02455c8f450804fcd35fd4,

title = "Multiple myeloma shows no intra-disease clustering of immunoglobulin heavy chain genes",

abstract = "BACKGROUND: Characterization of the immunoglobulin gene repertoire has improved our understanding of the immunopathogenesis of lymphoid tumors. Early B-lymphocyte precursors of multiple myeloma are known to exist and might be susceptible to antigenic drive. DESIGN AND METHODS: To verify this hypothesis, we collected a database of 345 fully readable multiple myeloma immunoglobulin sequences. We characterized the immunoglobulin repertoire, analyzed the somatic hypermutation load, and investigated for stereotyped receptor clusters. RESULTS: Compared to the normal immunoglobulin repertoire, multiple myeloma displayed only modest differences involving only a few genes, showing that the myeloma immunoglobulin repertoire is the least skewed among mature B-cell tumors. Median somatic hypermutation load was 7.8\%; median length of complementarity determining-region 3 was 15.5 amino acids. Clustering analysis showed the absence of myeloma specific clusters and no similarity with published chronic lymphocytic leukemia or lymphoma subsets. CONCLUSIONS: Analysis of multiple myeloma immunoglobulin repertoire does not support a pathogenetic role for antigen selection in this tumor.",

author = "S Ferrero and Daniela CAPELLO and M Svaldi and M Boi and D Gatti and D Drandi and Davide ROSSI and S Barbiero and B Mantoan and E Mantella and M Zanni and P Ghione and A Larocca and R Passera and F Bertoni and V Gattei and F Forconi and L Laurenti and Del, \{Poeta G\} and R Marasca and S Cortelazzo and Gianluca GAIDANO and A Palumbo and M Boccadoro and Marco LADETTO",

year = "2012",

doi = "10.3324/haematol.2011.052852",

language = "English",

volume = "97",

pages = "849--853",

journal = "Haematologica",

issn = "0390-6078",

publisher = "Ferrata Storti Foundation",

number = "6",

}

Ferrero, S, CAPELLO, D, Svaldi, M, Boi, M, Gatti, D, Drandi, D, ROSSI, D, Barbiero, S, Mantoan, B, Mantella, E, Zanni, M, Ghione, P, Larocca, A, Passera, R, Bertoni, F, Gattei, V, Forconi, F, Laurenti, L, Del, PG, Marasca, R, Cortelazzo, S, GAIDANO, G, Palumbo, A, Boccadoro, M & LADETTO, M 2012, 'Multiple myeloma shows no intra-disease clustering of immunoglobulin heavy chain genes', Haematologica, vol. 97, n. 6, pagg. 849-853. https://doi.org/10.3324/haematol.2011.052852

TY - JOUR

T1 - Multiple myeloma shows no intra-disease clustering of immunoglobulin heavy chain genes

AU - Ferrero, S

AU - CAPELLO, Daniela

AU - Svaldi, M

AU - Boi, M

AU - Gatti, D

AU - Drandi, D

AU - ROSSI, Davide

AU - Barbiero, S

AU - Mantoan, B

AU - Mantella, E

AU - Zanni, M

AU - Ghione, P

AU - Larocca, A

AU - Passera, R

AU - Bertoni, F

AU - Gattei, V

AU - Forconi, F

AU - Laurenti, L

AU - Del, Poeta G

AU - Marasca, R

AU - Cortelazzo, S

AU - GAIDANO, Gianluca

AU - Palumbo, A

AU - Boccadoro, M

AU - LADETTO, Marco

PY - 2012

Y1 - 2012

N2 - BACKGROUND: Characterization of the immunoglobulin gene repertoire has improved our understanding of the immunopathogenesis of lymphoid tumors. Early B-lymphocyte precursors of multiple myeloma are known to exist and might be susceptible to antigenic drive. DESIGN AND METHODS: To verify this hypothesis, we collected a database of 345 fully readable multiple myeloma immunoglobulin sequences. We characterized the immunoglobulin repertoire, analyzed the somatic hypermutation load, and investigated for stereotyped receptor clusters. RESULTS: Compared to the normal immunoglobulin repertoire, multiple myeloma displayed only modest differences involving only a few genes, showing that the myeloma immunoglobulin repertoire is the least skewed among mature B-cell tumors. Median somatic hypermutation load was 7.8%; median length of complementarity determining-region 3 was 15.5 amino acids. Clustering analysis showed the absence of myeloma specific clusters and no similarity with published chronic lymphocytic leukemia or lymphoma subsets. CONCLUSIONS: Analysis of multiple myeloma immunoglobulin repertoire does not support a pathogenetic role for antigen selection in this tumor.

AB - BACKGROUND: Characterization of the immunoglobulin gene repertoire has improved our understanding of the immunopathogenesis of lymphoid tumors. Early B-lymphocyte precursors of multiple myeloma are known to exist and might be susceptible to antigenic drive. DESIGN AND METHODS: To verify this hypothesis, we collected a database of 345 fully readable multiple myeloma immunoglobulin sequences. We characterized the immunoglobulin repertoire, analyzed the somatic hypermutation load, and investigated for stereotyped receptor clusters. RESULTS: Compared to the normal immunoglobulin repertoire, multiple myeloma displayed only modest differences involving only a few genes, showing that the myeloma immunoglobulin repertoire is the least skewed among mature B-cell tumors. Median somatic hypermutation load was 7.8%; median length of complementarity determining-region 3 was 15.5 amino acids. Clustering analysis showed the absence of myeloma specific clusters and no similarity with published chronic lymphocytic leukemia or lymphoma subsets. CONCLUSIONS: Analysis of multiple myeloma immunoglobulin repertoire does not support a pathogenetic role for antigen selection in this tumor.

UR - https://iris.uniupo.it/handle/11579/31441

U2 - 10.3324/haematol.2011.052852

DO - 10.3324/haematol.2011.052852

M3 - Article

SN - 0390-6078

VL - 97

SP - 849

EP - 853

JO - Haematologica

JF - Haematologica

IS - 6

ER -

Multiple myeloma shows no intra-disease clustering of immunoglobulin heavy chain genes

Abstract

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