Mouse Satellite Cell Isolation and Transplantation

ELIA ANGELINO; Simone Reano; Michele Ferrara; Emanuela Agosti; Hana Sustova; VALERIA MALACARNE; Sara Clerici; Andrea Graziani; Nicoletta FILIGHEDDU

doi:10.21769/BioProtoc.2696

Mouse Satellite Cell Isolation and Transplantation

ELIA ANGELINO, Simone Reano, Michele Ferrara, Emanuela Agosti, Hana Sustova, VALERIA MALACARNE, Sara Clerici, Andrea Graziani, Nicoletta FILIGHEDDU

Dipartimento di Medicina Traslazionale

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

: Satellite cell (SC) transplantation represents a powerful strategy to investigate SC biology during muscle regeneration. We described here a protocol for SC isolation from green fluorescent protein (GFP)-expressing mice and their transplantation into murine muscles. This procedure was originally used to assess the effects of the hormone unacylated ghrelin on muscle regeneration, in particular evaluating how the increase of unacylated ghrelin in the recipient muscle affected the engraftment of donor SCs ( Reano et al., 2017 ).

Lingua originale	Inglese
pagine (da-a)	e2696-N/A
Numero di pagine	13
Rivista	Bio-protocol
Volume	8
Numero di pubblicazione	2
DOI	https://doi.org/10.21769/BioProtoc.2696
Stato di pubblicazione	Pubblicato - 2018

Keywords

Cardiotoxin
Engraftment
Muscle excision
Muscle stem cells
Regeneration
Satellite cells
Skeletal muscle
Transplant

Accesso al documento

10.21769/BioProtoc.2696

https://hdl.handle.net/11579/172226

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title = "Mouse Satellite Cell Isolation and Transplantation",

abstract = ": Satellite cell (SC) transplantation represents a powerful strategy to investigate SC biology during muscle regeneration. We described here a protocol for SC isolation from green fluorescent protein (GFP)-expressing mice and their transplantation into murine muscles. This procedure was originally used to assess the effects of the hormone unacylated ghrelin on muscle regeneration, in particular evaluating how the increase of unacylated ghrelin in the recipient muscle affected the engraftment of donor SCs ( Reano et al., 2017 ).",

keywords = "Cardiotoxin, Engraftment, Muscle excision, Muscle stem cells, Regeneration, Satellite cells, Skeletal muscle, Transplant, Cardiotoxin, Engraftment, Muscle excision, Muscle stem cells, Regeneration, Satellite cells, Skeletal muscle, Transplant",

author = "ELIA ANGELINO and Simone Reano and Michele Ferrara and Emanuela Agosti and Hana Sustova and VALERIA MALACARNE and Sara Clerici and Andrea Graziani and Nicoletta FILIGHEDDU",

year = "2018",

doi = "10.21769/BioProtoc.2696",

language = "English",

volume = "8",

pages = "e2696--N/A",

journal = "Bio-protocol",

issn = "2331-8325",

publisher = "Bio-protocol LLC",

number = "2",

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TY - JOUR

T1 - Mouse Satellite Cell Isolation and Transplantation

AU - ANGELINO, ELIA

AU - Reano, Simone

AU - Ferrara, Michele

AU - Agosti, Emanuela

AU - Sustova, Hana

AU - MALACARNE, VALERIA

AU - Clerici, Sara

AU - Graziani, Andrea

AU - FILIGHEDDU, Nicoletta

PY - 2018

Y1 - 2018

N2 - : Satellite cell (SC) transplantation represents a powerful strategy to investigate SC biology during muscle regeneration. We described here a protocol for SC isolation from green fluorescent protein (GFP)-expressing mice and their transplantation into murine muscles. This procedure was originally used to assess the effects of the hormone unacylated ghrelin on muscle regeneration, in particular evaluating how the increase of unacylated ghrelin in the recipient muscle affected the engraftment of donor SCs ( Reano et al., 2017 ).

AB - : Satellite cell (SC) transplantation represents a powerful strategy to investigate SC biology during muscle regeneration. We described here a protocol for SC isolation from green fluorescent protein (GFP)-expressing mice and their transplantation into murine muscles. This procedure was originally used to assess the effects of the hormone unacylated ghrelin on muscle regeneration, in particular evaluating how the increase of unacylated ghrelin in the recipient muscle affected the engraftment of donor SCs ( Reano et al., 2017 ).

KW - Cardiotoxin

KW - Engraftment

KW - Muscle excision

KW - Muscle stem cells

KW - Regeneration

KW - Satellite cells

KW - Skeletal muscle

KW - Transplant

KW - Cardiotoxin

KW - Engraftment

KW - Muscle excision

KW - Muscle stem cells

KW - Regeneration

KW - Satellite cells

KW - Skeletal muscle

KW - Transplant

UR - https://iris.uniupo.it/handle/11579/172226

U2 - 10.21769/BioProtoc.2696

DO - 10.21769/BioProtoc.2696

M3 - Article

SN - 2331-8325

VL - 8

SP - e2696-N/A

JO - Bio-protocol

JF - Bio-protocol

IS - 2

ER -

Mouse Satellite Cell Isolation and Transplantation

Abstract

Keywords

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