Modulation of Oxidative Stress by 17 β-Estradiol and Genistein in Human Hepatic Cell Lines in Vitro

Daniela Surico, Alfredo Ercoli, Serena Farruggio, Giulia Raina, Davide Filippini, David Mary, Rosalba Minisini, Nicola Surico, Mario Pirisi, Elena Grossini

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

Background/Aims: estrogens and phytoestrogens exert hepatoprotection through mechanisms not clearly examined yet. Here, we investigated the protective effects exerted by 17β-estradiol and genistein against oxidative stress in hepatocytes and hepatic stellate cells (HSCs) and the involvement of specific receptors and the intracellular signalling. Methods: Huh7.5 and LX-2, alone or in co-culture with Huh7.5, were treated with 17β-estradiol and genistein alone or in the presence of menadione and of estrogen receptors (ERs) and G protein-coupled-estrogenic-receptors (GPER) blockers. Cell viability, mitochondrial membrane potential and oxidant/antioxidant system were measured by specific kits. Western Blot was used for the analysis of Akt and p38-mitogen-activated-protein kinases (MAPK) activation and α-smooth-muscle actin expression. Results: In Huh7.5, 17β-estradiol and genistein prevented the effects of peroxidation by modulating Akt and p38MAPK activation. Similar antioxidant and protective findings were obtained in LX-2 of co-culture experiments, only. ERs and GPER blockers were able to prevent the effects of 17β-estradiol and genistein. Conclusion: In Huh7.5 and LX-2, 17β-estradiol and genistein counteract the effects of peroxidation through the involvement of ERs and GPER and by an intracellular signalling related to Akt and p38MAPK. As concerning LX-2, paracrine factors released by Huh7.5 play a key role in protection against oxidative stress.

Lingua originaleInglese
pagine (da-a)1051-1062
Numero di pagine12
RivistaCellular Physiology and Biochemistry
Volume42
Numero di pubblicazione3
DOI
Stato di pubblicazionePubblicato - 28 giu 2017

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