Modulation by protein kinase C of the enhanced responsiveness to tachykinins in ovalbumin-sensitized guinea pig alveolar macrophages

S. Brunelleschi, S. Guidotto, E. Tonso, I. Viano, R. Fantozzi

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

As previously reported, alveolar macrophages (AMs) from ovalbumin- sensitized guinea pigs present an enhanced responsiveness to tachykinins but not to N-formylmethionyl-leucyl-phenylalanine (fMLP). We have investigated the biochemical mechanisms underlying this varied responsiveness to tachykinins. The protein kinase C (PKC) activator phorbol 12-myristate 13- acetate (PMA) induced a larger superoxide anion (O2/-) production in AMs from sensitized guinea pigs, as did tachykinins. Pretreatment of AMs with pertussis toxin abolished tachykinin-evoked respiratory burst, had no effect on PMA-evoked O2- production and strongly inhibited fMLP-evoked one, with no appreciable variation between control or sensitized AMs. Staurosporine and its derivative cgp 41251, significantly decreased PMA- and tachykinin- evoked O2/- production in both populations, being more potent in control AMs, but exerted little effects against fMLP. Pretreatment of AMs with PMA significantly inhibited fMLP-, PMA- and tachykinin-evoked O2/- production in both control and sensitized AMs. fMLP, substance P (SP), neurokinin A (NKA) and the NK2 agonist [β-Ala8]-NKA(4-10) dose-dependently increased [3H] phorbor 12, 13 dibutyrate (PDBu) binding to control and sensitized AMs. While fMLP exerted similar effects in both populations, dose-response curves for SP, NKA and the NK2 receptor agonist were shifted leftwards (1, 4 and 3 orders of magnitude, respectively) in sensitized AMs. These results indicate a possible PKC involvement in the enhanced responsiveness to tachykinins in actively sensitized AMs.

Lingua originaleInglese
pagine (da-a)249-260
Numero di pagine12
RivistaNeuropeptides
Volume30
Numero di pubblicazione3
DOI
Stato di pubblicazionePubblicato - 1996
Pubblicato esternamente

Fingerprint

Entra nei temi di ricerca di 'Modulation by protein kinase C of the enhanced responsiveness to tachykinins in ovalbumin-sensitized guinea pig alveolar macrophages'. Insieme formano una fingerprint unica.

Cita questo