TY - CHAP
T1 - Milk fat globule membrane components-a proteomic approach
AU - Cavaletto, Maria
AU - Giuffrida, Maria Gabriella
AU - Conti, Amedeo
PY - 2008
Y1 - 2008
N2 - The milk fat globule membrane (MFGM) is the membrane surrounding lipid droplets during their secretion in the alveolar lumen of the lactating mammary gland. MFGM proteins represent only 1-4% of total milk protein content; nevertheless, the MFGM consists of a complex system of integral and peripheral proteins, enzymes, and lipids. Despite their low classical nutritional value, MFGMproteins have been reported to play an important role in various cellular processes and defense mechanisms in the newborn. Using a proteomic approach, such as high-resolution, two-dimensional electrophoresis followed by direct protein identification by mass spectrometry, it has been possible to comprehensively characterize the subcellular organization of MFGM. This chapter covers the description of MFGM proteomics from the first studies about 10 years ago through the most recent papers. Most of the investigations deal with MFGMs from human and cow milk.
AB - The milk fat globule membrane (MFGM) is the membrane surrounding lipid droplets during their secretion in the alveolar lumen of the lactating mammary gland. MFGM proteins represent only 1-4% of total milk protein content; nevertheless, the MFGM consists of a complex system of integral and peripheral proteins, enzymes, and lipids. Despite their low classical nutritional value, MFGMproteins have been reported to play an important role in various cellular processes and defense mechanisms in the newborn. Using a proteomic approach, such as high-resolution, two-dimensional electrophoresis followed by direct protein identification by mass spectrometry, it has been possible to comprehensively characterize the subcellular organization of MFGM. This chapter covers the description of MFGM proteomics from the first studies about 10 years ago through the most recent papers. Most of the investigations deal with MFGMs from human and cow milk.
UR - http://www.scopus.com/inward/record.url?scp=84934439187&partnerID=8YFLogxK
U2 - 10.1007/978-0-387-74087-4_4
DO - 10.1007/978-0-387-74087-4_4
M3 - Chapter
SN - 9780387740867
T3 - Advances in Experimental Medicine and Biology
SP - 129
EP - 141
BT - Bioactive Components of Milk
PB - Springer New York
ER -