Abstract
The present work shows that the exposure of mesothelial cells to simulated microgravity changes their cytoskeleton and adhesion proteins, leading to a cell switch from normal towards tumoral cells. Immunohistochemical and molecular data were obtained from both MeT-5A exposed to simulated microgravity and BR95 mesothelioma cell lines. Simulated microgravity was found to affect the expression of actin, vinculin, and connexin-43, altering their quantitative and spatial distribution pattern inside the cell. The analysis of the tumoral markers p27, CD44, Fibulin-3, and NANOG and the expression of genes related to cancer transformation such as NANOG, CDH-1, and Zeb-1 showed that the simulated microgravity environment led to expression patterns in MeT-5A cells similar to those observed in BR95 cells. The alteration in both quantitative expression and structural organization of the cytoskeleton and adhesion/communication proteins can thus be considered a pivotal mechanism involved in the cellular shift towards tumoral progression.
Lingua originale | Inglese |
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pagine (da-a) | 10896-10912 |
Numero di pagine | 17 |
Rivista | Current Issues in Molecular Biology |
Volume | 46 |
Numero di pubblicazione | 10 |
DOI | |
Stato di pubblicazione | Pubblicato - 2024 |
Keywords
- BR95 cells
- connexin-43
- Fibulin-3
- focal contacts
- mesothelioma
- MeT-5A cells
- NANOG