TY - JOUR
T1 - Lymphocyte Na,K-ATPase is reduced in aged people
AU - Bozzo, Chiarella
AU - Goria, Maria
AU - Marengo, Claudio
AU - Marena, Saverio
AU - Veglia, Fabrizio
AU - Pagano, Gianfranco
N1 - Funding Information:
From the Institute of Internal Medicine, University Italy. Supported by a grant from the Minister0 Pubblica (MPI60% 1987). Address reprint requests to Gianfranco Pagano, MD, Medicina Interna. Universitb di Torino, Corso Polonia Torino. Italy. a 1990 by W.B. Saunders Company. 00260495/90/3908-0005$03.00/0
PY - 1990/8
Y1 - 1990/8
N2 - Na,K-ATPase-dependent 86Rb uptake, maximum velocity (Vmax), Michaelis constant (Km) of the uptake, and [3H]-ouabain binding were investigated in the lymphocytes of 10 elderly subjects (age > 60 years), and in 10 middle-aged (41 to 60 years) and 10 young controls (age ≤ 40 years). 86Rb uptake was reduced in elderly versus both middle-aged and young subjects (20.14 ± 3.30 v 35.60 ± 2.67, P = .002, and v 36.53 ± 4.49 nmol, P = .012), as was the number of [3H]-ouabain binding sites per cell (32,662 ± 2,215 v 40,420 ± 1,184, P = .011, and v 40,596 ± 1,349, P = .014). Vmax was reduced in elderly v young subjects (1.20 ± 0.10 v 1.64 ± 0.13, P = .034), but not versus the middle-age group (1.20 ± 0.10 v 1.54 ± 0.12 nmol · min-1, NS). Km was no different among the three groups. No differences were found between middle-aged and young subjects. Significant correlations were observed between age and Na,K-ATPase-dependent 86Rb uptake (r = -.620, P = .00009), Vmax (r = -.439, P = .024), and [3H]-ouabain binding sites (r = -.648, P = .002). Moreover, the site number was positively correlated with both uptake (r = .635, P = .002) and Vmax (r = .554, P = .011). These differences were observed both in women and men. We conclude that there is an age-dependent reduction in lymphocyte Na,K-ATPase activity, which is fully manifested over 60 years, and that this alteration is probably due to the reduced number of functional units of Na,K-ATPase in advancing age.
AB - Na,K-ATPase-dependent 86Rb uptake, maximum velocity (Vmax), Michaelis constant (Km) of the uptake, and [3H]-ouabain binding were investigated in the lymphocytes of 10 elderly subjects (age > 60 years), and in 10 middle-aged (41 to 60 years) and 10 young controls (age ≤ 40 years). 86Rb uptake was reduced in elderly versus both middle-aged and young subjects (20.14 ± 3.30 v 35.60 ± 2.67, P = .002, and v 36.53 ± 4.49 nmol, P = .012), as was the number of [3H]-ouabain binding sites per cell (32,662 ± 2,215 v 40,420 ± 1,184, P = .011, and v 40,596 ± 1,349, P = .014). Vmax was reduced in elderly v young subjects (1.20 ± 0.10 v 1.64 ± 0.13, P = .034), but not versus the middle-age group (1.20 ± 0.10 v 1.54 ± 0.12 nmol · min-1, NS). Km was no different among the three groups. No differences were found between middle-aged and young subjects. Significant correlations were observed between age and Na,K-ATPase-dependent 86Rb uptake (r = -.620, P = .00009), Vmax (r = -.439, P = .024), and [3H]-ouabain binding sites (r = -.648, P = .002). Moreover, the site number was positively correlated with both uptake (r = .635, P = .002) and Vmax (r = .554, P = .011). These differences were observed both in women and men. We conclude that there is an age-dependent reduction in lymphocyte Na,K-ATPase activity, which is fully manifested over 60 years, and that this alteration is probably due to the reduced number of functional units of Na,K-ATPase in advancing age.
UR - https://www.scopus.com/pages/publications/0024997094
U2 - 10.1016/0026-0495(90)90123-T
DO - 10.1016/0026-0495(90)90123-T
M3 - Article
SN - 0026-0495
VL - 39
SP - 808
EP - 814
JO - Metabolism: Clinical and Experimental
JF - Metabolism: Clinical and Experimental
IS - 8
ER -
