LPS-inducible nuclear factor in human monocytes that binds the negative regulatory element of the HIV LTR

T. Musso; M. C. Bosco; D. L. Longo; I. Espinoza-Delgado; A. Sica; G. W. Cox; G. L. Gusella; G. Forni; L. Varesio

doi:10.1002/jlb.56.1.21

LPS-inducible nuclear factor in human monocytes that binds the negative regulatory element of the HIV LTR

T. Musso, M. C. Bosco, D. L. Longo, I. Espinoza-Delgado, A. Sica, G. W. Cox, G. L. Gusella, G. Forni, L. Varesio

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

We studied the constitutive and lipopolysaccharide (LPS)-induced expression of nuclear protein binding to the negative regulatory element (NRE) of the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) in fresh human monocytes. We demonstrated the existence of a constitutive factor binding to the NRE 73-bp HpaII/HpaII fragment (-216 to -143) whose expression is up-regulated by LPS treatment. Competition experiments with overlapping oligonucleotides covering the NpaII/HpaII fragment and with mutated oligonucleotides mapped the binding within the TTTCATCAC region (-171 to -163). This binding pattern is unique to human monocytes.

Lingua originale	Inglese
pagine (da-a)	21-26
Numero di pagine	6
Rivista	Journal of Leukocyte Biology
Volume	56
Numero di pubblicazione	1
DOI	https://doi.org/10.1002/jlb.56.1.21
Stato di pubblicazione	Pubblicato - 1994
Pubblicato esternamente	Sì

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10.1002/jlb.56.1.21

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title = "LPS-inducible nuclear factor in human monocytes that binds the negative regulatory element of the HIV LTR",

abstract = "We studied the constitutive and lipopolysaccharide (LPS)-induced expression of nuclear protein binding to the negative regulatory element (NRE) of the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) in fresh human monocytes. We demonstrated the existence of a constitutive factor binding to the NRE 73-bp HpaII/HpaII fragment (-216 to -143) whose expression is up-regulated by LPS treatment. Competition experiments with overlapping oligonucleotides covering the NpaII/HpaII fragment and with mutated oligonucleotides mapped the binding within the TTTCATCAC region (-171 to -163). This binding pattern is unique to human monocytes.",

keywords = "HIV, Human monocytes, Lipopolysaccharide, Nuclear factor",

author = "T. Musso and Bosco, \{M. C.\} and Longo, \{D. L.\} and I. Espinoza-Delgado and A. Sica and Cox, \{G. W.\} and Gusella, \{G. L.\} and G. Forni and L. Varesio",

year = "1994",

doi = "10.1002/jlb.56.1.21",

language = "English",

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T1 - LPS-inducible nuclear factor in human monocytes that binds the negative regulatory element of the HIV LTR

AU - Musso, T.

AU - Bosco, M. C.

AU - Longo, D. L.

AU - Espinoza-Delgado, I.

AU - Sica, A.

AU - Cox, G. W.

AU - Gusella, G. L.

AU - Forni, G.

AU - Varesio, L.

PY - 1994

Y1 - 1994

N2 - We studied the constitutive and lipopolysaccharide (LPS)-induced expression of nuclear protein binding to the negative regulatory element (NRE) of the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) in fresh human monocytes. We demonstrated the existence of a constitutive factor binding to the NRE 73-bp HpaII/HpaII fragment (-216 to -143) whose expression is up-regulated by LPS treatment. Competition experiments with overlapping oligonucleotides covering the NpaII/HpaII fragment and with mutated oligonucleotides mapped the binding within the TTTCATCAC region (-171 to -163). This binding pattern is unique to human monocytes.

AB - We studied the constitutive and lipopolysaccharide (LPS)-induced expression of nuclear protein binding to the negative regulatory element (NRE) of the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) in fresh human monocytes. We demonstrated the existence of a constitutive factor binding to the NRE 73-bp HpaII/HpaII fragment (-216 to -143) whose expression is up-regulated by LPS treatment. Competition experiments with overlapping oligonucleotides covering the NpaII/HpaII fragment and with mutated oligonucleotides mapped the binding within the TTTCATCAC region (-171 to -163). This binding pattern is unique to human monocytes.

KW - HIV

KW - Human monocytes

KW - Lipopolysaccharide

KW - Nuclear factor

UR - https://www.scopus.com/pages/publications/0028100409

U2 - 10.1002/jlb.56.1.21

DO - 10.1002/jlb.56.1.21

M3 - Article

SN - 0741-5400

VL - 56

SP - 21

EP - 26

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

IS - 1

ER -

LPS-inducible nuclear factor in human monocytes that binds the negative regulatory element of the HIV LTR

Abstract

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