La citofluorimetria a flusso può risolvere i problemi di misura dell'aerosol fungino?

Current limitations in the methodology for enumeration of airborne fungal propagules compromise the precision and accuracy of bioaerosol exposure assessment. Flow cytometry (FCM) is a tool with great potential for use in environmental microbiology because of the quantity and quality of data it provides in a timely fashion. In this study, FCM was used to count fungal propagules in field samples. To verify the accuracy and the precision of this technique, fungal propagules counts made by flow cytometry were compared with counts by direct observation using epifluorescence microscopy. Field samples were stained with propidium iodide after microwave irradiation, to discriminate the biological particles, while forward angle light scattering was used for identifying and counting fungal propagules population. A close agreement was found between FCM and epifluorescence microscopy counts.