Isolation and characterization of full and truncated forms of human breast carcinoma protein BA46 from human milk fat globule membranes

Maria Gabriella Giuffrida; Maria Cavaletto; Carlo Giunta; Amedeo Conti; Jasminka Godovac-Zimmermann

doi:10.1023/A:1022531500370

Isolation and characterization of full and truncated forms of human breast carcinoma protein BA46 from human milk fat globule membranes

Maria Gabriella Giuffrida, Maria Cavaletto, Carlo Giunta, Amedeo Conti, Jasminka Godovac-Zimmermann

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

We have isolated and characterized two proteins of 50 and 30 kDa from human milk fat globule membranes of healthy donors. N-terminal and internal sequencing revealed that the 50-kDa protein is the full-length human breast carcinoma protein BA46 that is highly expressed in human breast tumors. The 30-kDa protein is a truncated form of protein BA46 which consists of the C- terminal factor V/VIII-like domain of BA46 and which appears to anchor BA46 to the milk fat globule membrane. Defective release of the epidermal growth factor domain containing a surface RGD motif may be related to involvement of BA46 in breast cancer.

Lingua originale	Inglese
pagine (da-a)	143-148
Numero di pagine	6
Rivista	Journal of Protein Chemistry
Volume	17
Numero di pubblicazione	2
DOI	https://doi.org/10.1023/A:1022531500370
Stato di pubblicazione	Pubblicato - 1998
Pubblicato esternamente	Sì

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title = "Isolation and characterization of full and truncated forms of human breast carcinoma protein BA46 from human milk fat globule membranes",

abstract = "We have isolated and characterized two proteins of 50 and 30 kDa from human milk fat globule membranes of healthy donors. N-terminal and internal sequencing revealed that the 50-kDa protein is the full-length human breast carcinoma protein BA46 that is highly expressed in human breast tumors. The 30-kDa protein is a truncated form of protein BA46 which consists of the C- terminal factor V/VIII-like domain of BA46 and which appears to anchor BA46 to the milk fat globule membrane. Defective release of the epidermal growth factor domain containing a surface RGD motif may be related to involvement of BA46 in breast cancer.",

keywords = "Epidermal growth factor, Factor V/VIII, Human breast carcinoma, Human milk fat globule membrane proteins",

author = "Giuffrida, {Maria Gabriella} and Maria Cavaletto and Carlo Giunta and Amedeo Conti and Jasminka Godovac-Zimmermann",

year = "1998",

doi = "10.1023/A:1022531500370",

language = "English",

volume = "17",

pages = "143--148",

journal = "Journal of Protein Chemistry",

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T1 - Isolation and characterization of full and truncated forms of human breast carcinoma protein BA46 from human milk fat globule membranes

AU - Giuffrida, Maria Gabriella

AU - Cavaletto, Maria

AU - Giunta, Carlo

AU - Conti, Amedeo

AU - Godovac-Zimmermann, Jasminka

PY - 1998

Y1 - 1998

N2 - We have isolated and characterized two proteins of 50 and 30 kDa from human milk fat globule membranes of healthy donors. N-terminal and internal sequencing revealed that the 50-kDa protein is the full-length human breast carcinoma protein BA46 that is highly expressed in human breast tumors. The 30-kDa protein is a truncated form of protein BA46 which consists of the C- terminal factor V/VIII-like domain of BA46 and which appears to anchor BA46 to the milk fat globule membrane. Defective release of the epidermal growth factor domain containing a surface RGD motif may be related to involvement of BA46 in breast cancer.

AB - We have isolated and characterized two proteins of 50 and 30 kDa from human milk fat globule membranes of healthy donors. N-terminal and internal sequencing revealed that the 50-kDa protein is the full-length human breast carcinoma protein BA46 that is highly expressed in human breast tumors. The 30-kDa protein is a truncated form of protein BA46 which consists of the C- terminal factor V/VIII-like domain of BA46 and which appears to anchor BA46 to the milk fat globule membrane. Defective release of the epidermal growth factor domain containing a surface RGD motif may be related to involvement of BA46 in breast cancer.

KW - Epidermal growth factor

KW - Factor V/VIII

KW - Human breast carcinoma

KW - Human milk fat globule membrane proteins

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U2 - 10.1023/A:1022531500370

DO - 10.1023/A:1022531500370

M3 - Article

SN - 0277-8033

VL - 17

SP - 143

EP - 148

JO - Journal of Protein Chemistry

JF - Journal of Protein Chemistry

IS - 2

ER -

Isolation and characterization of full and truncated forms of human breast carcinoma protein BA46 from human milk fat globule membranes

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