TY - JOUR
T1 - Ions and water transmembrane transport in nervous and testicular cultured cells in low gravity conditions
AU - Uva, Bianca Maria
AU - Strollo, Felice
AU - Ricci, Franco
AU - Pastorino, Martina
AU - Tagliaferro, Grazia
AU - Mariani, Maria Rita
AU - Masini, Maria Angela
PY - 2006
Y1 - 2006
N2 - Aim of the present study was to investigate on the possible alterations induced by on ground modeled microgravity on ion-water transport proteins at cellular level For the purpose we used astrocytes, C6 line, neurons (NT2 line from human teratocarcinoma) and testicular cells (germ cells, Sertoli cells, and Leydig cells; primary cultures from trypsinised prepuberal pig testes). Modeled microgravity was achieved by a desktop 3D Random Positioning Machine, cultures were kept rotating for 30', Ih and 24h. After 30', immunopositivity for the antibodies to Na+/K+ATPase and Na+/K+/Cl- co-transporters was greatly diminished, the plasma membrane appeared to be altered, and the mitochondria inner cristae were disrupted. Immunostaining to the antibody to the water channel aquaporin 4 was very bright. After 1h at random rotation immunostaining for the heat shock protein Hsp27 was visible, After 24h, immunostaining for the ion transport proteins was again like that of the controls, plasma membrane and the mitochondria were again normal. Immunostaining for aquaporin 4 become again similar to that of the controls. We conclude that low gravity induces only transient alterations in the cell's transmembrane ion-water transport: the cells are able to adapt to the gravity vector changes in few hours.
AB - Aim of the present study was to investigate on the possible alterations induced by on ground modeled microgravity on ion-water transport proteins at cellular level For the purpose we used astrocytes, C6 line, neurons (NT2 line from human teratocarcinoma) and testicular cells (germ cells, Sertoli cells, and Leydig cells; primary cultures from trypsinised prepuberal pig testes). Modeled microgravity was achieved by a desktop 3D Random Positioning Machine, cultures were kept rotating for 30', Ih and 24h. After 30', immunopositivity for the antibodies to Na+/K+ATPase and Na+/K+/Cl- co-transporters was greatly diminished, the plasma membrane appeared to be altered, and the mitochondria inner cristae were disrupted. Immunostaining to the antibody to the water channel aquaporin 4 was very bright. After 1h at random rotation immunostaining for the heat shock protein Hsp27 was visible, After 24h, immunostaining for the ion transport proteins was again like that of the controls, plasma membrane and the mitochondria were again normal. Immunostaining for aquaporin 4 become again similar to that of the controls. We conclude that low gravity induces only transient alterations in the cell's transmembrane ion-water transport: the cells are able to adapt to the gravity vector changes in few hours.
UR - http://www.scopus.com/inward/record.url?scp=33845392763&partnerID=8YFLogxK
U2 - 10.1007/BF02870418
DO - 10.1007/BF02870418
M3 - Article
SN - 0938-0108
VL - 18
SP - 239
EP - 242
JO - Microgravity Science and Technology
JF - Microgravity Science and Technology
IS - 3-4
ER -