TY - JOUR
T1 - Ion transport proteins and aquaporin water channels in the kidney of amphibians from different habitats
AU - Sturla, M.
AU - Prato, P.
AU - Masini, M. A.
AU - Uva, B. M.
N1 - Funding Information:
This work was supported by the University of Genoa, Italy. The antibody developed by Fambrough D.M. was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biological Sciences, Iowa City, IA 52242.
PY - 2003/9
Y1 - 2003/9
N2 - Amphibians are known to spend part of their life on land and return to water to reproduce. However, some urodeles spend their entire life in water, while others succeed in completely avoiding water even during reproduction. Osmoregulatory mechanisms must therefore be different in the diverse environmental conditions of their respective life histories. The architecture of the kidney is similar in all amphibians; as a consequence the ion-water equilibrium must be regulated in the different environmental conditions. We investigated the immunolocalisation of Na+/K+/Cl - cotransport proteins, sodium pump and water-channel proteins (aquaporins) in aquatic Amphiuma means means, Rana dalmatina, a species that returns to water to reproduce, and Speleomantes genei, a completely terrestrial species. The investigation was carried out with immunohistochemical methods using antibodies to Na+/K+/Cl- cotransport protein NKCC1 T4, Na+/K+ATPase α-subunit, water-channel aquaporin 3 and the inner mitochondrial membrane (AMA). Cotransport proteins and sodium pump, involved in ion reabsorption, are widely distributed in A. means and R. dalmatina and confined to the distal segment in S. genei; conversely water channels, involved in water reabsorption, are limited to the collecting duct in A. means and R. dalmatina and distributed in the proximal and collecting ducts in S. genei.
AB - Amphibians are known to spend part of their life on land and return to water to reproduce. However, some urodeles spend their entire life in water, while others succeed in completely avoiding water even during reproduction. Osmoregulatory mechanisms must therefore be different in the diverse environmental conditions of their respective life histories. The architecture of the kidney is similar in all amphibians; as a consequence the ion-water equilibrium must be regulated in the different environmental conditions. We investigated the immunolocalisation of Na+/K+/Cl - cotransport proteins, sodium pump and water-channel proteins (aquaporins) in aquatic Amphiuma means means, Rana dalmatina, a species that returns to water to reproduce, and Speleomantes genei, a completely terrestrial species. The investigation was carried out with immunohistochemical methods using antibodies to Na+/K+/Cl- cotransport protein NKCC1 T4, Na+/K+ATPase α-subunit, water-channel aquaporin 3 and the inner mitochondrial membrane (AMA). Cotransport proteins and sodium pump, involved in ion reabsorption, are widely distributed in A. means and R. dalmatina and confined to the distal segment in S. genei; conversely water channels, involved in water reabsorption, are limited to the collecting duct in A. means and R. dalmatina and distributed in the proximal and collecting ducts in S. genei.
KW - Amphibia
KW - Amphiuma
KW - Aquaporins
KW - Immunohistochemistry
KW - Ion cotransporter proteins
KW - Kidney
KW - Rana
KW - Sodium pump
KW - Speleomantes
UR - http://www.scopus.com/inward/record.url?scp=1542399872&partnerID=8YFLogxK
U2 - 10.1016/S1532-0456(03)00141-8
DO - 10.1016/S1532-0456(03)00141-8
M3 - Article
SN - 1532-0456
VL - 136
SP - 1
EP - 7
JO - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
JF - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
IS - 1
ER -