Ion transport proteins and aquaporin water channels in the kidney of amphibians from different habitats

M. Sturla, P. Prato, M. A. Masini, B. M. Uva

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

Amphibians are known to spend part of their life on land and return to water to reproduce. However, some urodeles spend their entire life in water, while others succeed in completely avoiding water even during reproduction. Osmoregulatory mechanisms must therefore be different in the diverse environmental conditions of their respective life histories. The architecture of the kidney is similar in all amphibians; as a consequence the ion-water equilibrium must be regulated in the different environmental conditions. We investigated the immunolocalisation of Na+/K+/Cl - cotransport proteins, sodium pump and water-channel proteins (aquaporins) in aquatic Amphiuma means means, Rana dalmatina, a species that returns to water to reproduce, and Speleomantes genei, a completely terrestrial species. The investigation was carried out with immunohistochemical methods using antibodies to Na+/K+/Cl- cotransport protein NKCC1 T4, Na+/K+ATPase α-subunit, water-channel aquaporin 3 and the inner mitochondrial membrane (AMA). Cotransport proteins and sodium pump, involved in ion reabsorption, are widely distributed in A. means and R. dalmatina and confined to the distal segment in S. genei; conversely water channels, involved in water reabsorption, are limited to the collecting duct in A. means and R. dalmatina and distributed in the proximal and collecting ducts in S. genei.

Lingua originaleInglese
pagine (da-a)1-7
Numero di pagine7
RivistaComparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Volume136
Numero di pubblicazione1
DOI
Stato di pubblicazionePubblicato - set 2003
Pubblicato esternamente

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