In vitro micronucleus induction by polymethyl methacrylate bone cement in cultured human lymphocytes

M. Paola Bigatti; Laura Lamberti; Francesco Paolo Rizzi; Mario Cannas; Giampietro Allasia

doi:10.1016/0165-1218(94)90037-X

In vitro micronucleus induction by polymethyl methacrylate bone cement in cultured human lymphocytes

M. Paola Bigatti, Laura Lamberti, Francesco Paolo Rizzi, Mario Cannas, Giampietro Allasia

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

Human lymphocytes cultured in vitro were used to assess the ability of polymethyl methacrylate (PMMA), currently used in orthopaedic surgery as bone cement, to induce micronuclei in binucleated cells. The results of the study show a significant increase in the micronucleus frequency in treated cultures and therefore the genotoxic effect of PMMA bone cement or its ingredients (methyl methacrylate, dimethyl para-toluidine and hydroquinone) usually present in self-curing methacrylate bone cement and released in small quantities after polymerisation. This effect is evident during the stage immediately after the polymerisation process, and after a certain period of time (5 days in our experimental model).

Lingua originale	Inglese
pagine (da-a)	133-137
Numero di pagine	5
Rivista	Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure
Volume	321
Numero di pubblicazione	3
DOI	https://doi.org/10.1016/0165-1218(94)90037-X
Stato di pubblicazione	Pubblicato - mag 1994
Pubblicato esternamente	Sì

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10.1016/0165-1218(94)90037-X

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@article{e4c03568f42946deacecd814a63e41b9,

title = "In vitro micronucleus induction by polymethyl methacrylate bone cement in cultured human lymphocytes",

abstract = "Human lymphocytes cultured in vitro were used to assess the ability of polymethyl methacrylate (PMMA), currently used in orthopaedic surgery as bone cement, to induce micronuclei in binucleated cells. The results of the study show a significant increase in the micronucleus frequency in treated cultures and therefore the genotoxic effect of PMMA bone cement or its ingredients (methyl methacrylate, dimethyl para-toluidine and hydroquinone) usually present in self-curing methacrylate bone cement and released in small quantities after polymerisation. This effect is evident during the stage immediately after the polymerisation process, and after a certain period of time (5 days in our experimental model).",

keywords = "In vitro test, Lymphocyte culture, Micronuclei, PMMA bone cement",

author = "Bigatti, {M. Paola} and Laura Lamberti and Rizzi, {Francesco Paolo} and Mario Cannas and Giampietro Allasia",

year = "1994",

month = may,

doi = "10.1016/0165-1218(94)90037-X",

language = "English",

volume = "321",

pages = "133--137",

journal = "Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure",

issn = "0165-1218",

publisher = "Elsevier B.V.",

number = "3",

}

In vitro micronucleus induction by polymethyl methacrylate bone cement in cultured human lymphocytes. / Bigatti, M. Paola; Lamberti, Laura; Rizzi, Francesco Paolo et al.
In: Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure, Vol. 321, N. 3, 05.1994, pag. 133-137.

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

TY - JOUR

T1 - In vitro micronucleus induction by polymethyl methacrylate bone cement in cultured human lymphocytes

AU - Bigatti, M. Paola

AU - Lamberti, Laura

AU - Rizzi, Francesco Paolo

AU - Cannas, Mario

AU - Allasia, Giampietro

PY - 1994/5

Y1 - 1994/5

N2 - Human lymphocytes cultured in vitro were used to assess the ability of polymethyl methacrylate (PMMA), currently used in orthopaedic surgery as bone cement, to induce micronuclei in binucleated cells. The results of the study show a significant increase in the micronucleus frequency in treated cultures and therefore the genotoxic effect of PMMA bone cement or its ingredients (methyl methacrylate, dimethyl para-toluidine and hydroquinone) usually present in self-curing methacrylate bone cement and released in small quantities after polymerisation. This effect is evident during the stage immediately after the polymerisation process, and after a certain period of time (5 days in our experimental model).

AB - Human lymphocytes cultured in vitro were used to assess the ability of polymethyl methacrylate (PMMA), currently used in orthopaedic surgery as bone cement, to induce micronuclei in binucleated cells. The results of the study show a significant increase in the micronucleus frequency in treated cultures and therefore the genotoxic effect of PMMA bone cement or its ingredients (methyl methacrylate, dimethyl para-toluidine and hydroquinone) usually present in self-curing methacrylate bone cement and released in small quantities after polymerisation. This effect is evident during the stage immediately after the polymerisation process, and after a certain period of time (5 days in our experimental model).

KW - In vitro test

KW - Lymphocyte culture

KW - Micronuclei

KW - PMMA bone cement

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U2 - 10.1016/0165-1218(94)90037-X

DO - 10.1016/0165-1218(94)90037-X

M3 - Article

SN - 0165-1218

VL - 321

SP - 133

EP - 137

JO - Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure

JF - Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure

IS - 3

ER -

In vitro micronucleus induction by polymethyl methacrylate bone cement in cultured human lymphocytes

Abstract

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