In vitro evidence for CCl₄ metabolites covalently bound to lipoprotein micelles

CCl₄-induced impairment of the lipoprotein secretion pathway of intact rat hepatocytes was carried out using ¹⁴CCl₄ to check the possibility of binding to lipoproteins by CCl₄ metabolites. After separation of different cell suspension fractions by means of ultracentrifugation and chemical precipitation procedures, a significant amount of the radioisotope was found covalently bound to the lipid and protein components of low density lipoproteins. Suitable experiments demonstrated that the bound radioisotope was represented by CCl₄ metabolites and not by unactivated CCl₄.