TY - JOUR
T1 - Immunoreactive localization of vasoactive hormones (atrial natriuretic peptide and endothelin) in the heart of Protopterus annectens, an African lungfish
AU - Masini, Maria Angela
AU - Sturla, Maddalena
AU - Napoli, Laura
AU - Uva, Bianca Maria
PY - 1996
Y1 - 1996
N2 - The present study demonstrated, by immunohistochemistry and Western blotting, the presence of immunoreacting atrial natriuretic peptide (ANP) and endothelin in the heart of Protopterus annectens by both light and electron microscopy. The distribution of ANP granules was investigated. ANP granules were localised in myocytes from the atrium, ventricle and conus arteriosus; endothelin-1 (ET-1) was demonstrated in subendocardial myocytes of the atrium and the conus. No ET-1 immunoreactivity was observed in the ventricle wall. At the light-microscopical level, ET-1 appeared to occur in the endocardium, but at the electron-microscopical level no immunogold labelling was seen on the granules of the endocardial cells. It is suggested that ET-1 is produced and stored in the subendocardial cells and released into the subendocardial space to reach the ANP-producing myocytes and the endothelial cells.
AB - The present study demonstrated, by immunohistochemistry and Western blotting, the presence of immunoreacting atrial natriuretic peptide (ANP) and endothelin in the heart of Protopterus annectens by both light and electron microscopy. The distribution of ANP granules was investigated. ANP granules were localised in myocytes from the atrium, ventricle and conus arteriosus; endothelin-1 (ET-1) was demonstrated in subendocardial myocytes of the atrium and the conus. No ET-1 immunoreactivity was observed in the ventricle wall. At the light-microscopical level, ET-1 appeared to occur in the endocardium, but at the electron-microscopical level no immunogold labelling was seen on the granules of the endocardial cells. It is suggested that ET-1 is produced and stored in the subendocardial cells and released into the subendocardial space to reach the ANP-producing myocytes and the endothelial cells.
KW - Atrial natriuretic peptide (ANP)
KW - Endothelin-1
KW - Heart
KW - Immunohistochemistry
KW - Protopterus annectens (Dipnoi)
UR - http://www.scopus.com/inward/record.url?scp=0029976747&partnerID=8YFLogxK
U2 - 10.1007/s004410050612
DO - 10.1007/s004410050612
M3 - Article
SN - 0302-766X
VL - 284
SP - 501
EP - 507
JO - Cell and Tissue Research
JF - Cell and Tissue Research
IS - 3
ER -