TY - JOUR
T1 - Identification of human cytomegalovirus strain with immediate-early (IE) antigen-specific monoclonal antibody is prevented by point mutation in IE gene
AU - Zipeto, D.
AU - Sarasini, A.
AU - Rossi, F.
AU - Baldanti, F.
AU - Revello, M. G.
AU - Milanesi, G.
AU - Gerna, G.
PY - 1994
Y1 - 1994
N2 - In an AIDS patient with a disseminated human cytomegalovirus (HCMV) infection, presence of HCMV in blood was repeatedly excluded by the shell vial culture method with the HCMV immediate-early (IE) antigen-specific monoclonal antibody (MAb) 5D2 currently employed for rapid HCMV identification, whereas it was repeatedly confirmed by all other assays (conventional virus isolation from blood, antigenemia, and DNAemia). Sequence analysis of the HCMV strain revealed a point mutation in exon 2 of the IE gene, which led to a serine-to-proline substitution at position 20 of the corresponding protein. Cloning and expression of a region of the IE gene containing the mutation showed that this was responsible for the lack of reactivity of MAb 5D2. A pool of IE antigen-reactive MAbs instead of a single MAb must be used for rapid HCMV identification to detect all viral strains.
AB - In an AIDS patient with a disseminated human cytomegalovirus (HCMV) infection, presence of HCMV in blood was repeatedly excluded by the shell vial culture method with the HCMV immediate-early (IE) antigen-specific monoclonal antibody (MAb) 5D2 currently employed for rapid HCMV identification, whereas it was repeatedly confirmed by all other assays (conventional virus isolation from blood, antigenemia, and DNAemia). Sequence analysis of the HCMV strain revealed a point mutation in exon 2 of the IE gene, which led to a serine-to-proline substitution at position 20 of the corresponding protein. Cloning and expression of a region of the IE gene containing the mutation showed that this was responsible for the lack of reactivity of MAb 5D2. A pool of IE antigen-reactive MAbs instead of a single MAb must be used for rapid HCMV identification to detect all viral strains.
UR - http://www.scopus.com/inward/record.url?scp=0028212381&partnerID=8YFLogxK
U2 - 10.1128/jcm.32.5.1402-1405.1994
DO - 10.1128/jcm.32.5.1402-1405.1994
M3 - Comment/debate
SN - 0095-1137
VL - 32
SP - 1402
EP - 1405
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 5
ER -