TY - JOUR
T1 - IκB-α, the NF-κB Inhibitory Subunit, Interacts with ANT, the Mitochondrial ATP/ADP Translocator
AU - Bottero, Virginie
AU - Rossi, Franca
AU - Samson, Michel
AU - Mari, Mireille
AU - Hofman, Paul
AU - Peyron, Jean François
PY - 2001/6/15
Y1 - 2001/6/15
N2 - The transcription factor NF-κB regulates a wide set of genes involved in the establishment of many cellular processes that control cell activation, proliferation, and apoptosis. IκB inhibitory subunits integrate NF-κB activation signals through phosphorylation and ubiquitination of its N-terminal domain. Using the two-hybrid system in yeast, we searched for IκB-α N-terminal domain interactors and therefore potential NF-κ-B regulators. An interaction of IκB-α with the mitochondrial ATP/ADP translocator ANT was detected in yeast and confirmed in glutathione S-transferase pull-down assays and co-precipitation experiments in transfected cells. Subcellular cell fractionation, resistance to proteinase K treatment, and electron microscopy experiments demonstrated the presence of IκB-α and associated p65 NF-κB in the mitochondrial intermembrane space. IκB-α·NF-κB appeared to be released from mitochondria upon the induction of apoptosis by engagement of the Fas receptor. These data suggest that the mitochondrial IκB-α ·NF-κB pool participates in the regulation of apoptosis.
AB - The transcription factor NF-κB regulates a wide set of genes involved in the establishment of many cellular processes that control cell activation, proliferation, and apoptosis. IκB inhibitory subunits integrate NF-κB activation signals through phosphorylation and ubiquitination of its N-terminal domain. Using the two-hybrid system in yeast, we searched for IκB-α N-terminal domain interactors and therefore potential NF-κ-B regulators. An interaction of IκB-α with the mitochondrial ATP/ADP translocator ANT was detected in yeast and confirmed in glutathione S-transferase pull-down assays and co-precipitation experiments in transfected cells. Subcellular cell fractionation, resistance to proteinase K treatment, and electron microscopy experiments demonstrated the presence of IκB-α and associated p65 NF-κB in the mitochondrial intermembrane space. IκB-α·NF-κB appeared to be released from mitochondria upon the induction of apoptosis by engagement of the Fas receptor. These data suggest that the mitochondrial IκB-α ·NF-κB pool participates in the regulation of apoptosis.
UR - http://www.scopus.com/inward/record.url?scp=0035877820&partnerID=8YFLogxK
U2 - 10.1074/jbc.M005850200
DO - 10.1074/jbc.M005850200
M3 - Article
SN - 0021-9258
VL - 276
SP - 21317
EP - 21324
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 24
ER -