TY - JOUR
T1 - Hyaluronated mesoporous silica nanoparticles for active targeting
T2 - influence of conjugation method and hyaluronic acid molecular weight on the nanovector properties
AU - Ricci, Valentina
AU - Zonari, Daniele
AU - Cannito, Stefania
AU - Marengo, Alessandro
AU - Scupoli, Maria Teresa
AU - Malatesta, Manuela
AU - Carton, Flavia
AU - Boschi, Federico
AU - Berlier, Gloria
AU - Arpicco, Silvia
N1 - Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2018/4/15
Y1 - 2018/4/15
N2 - We have prepared and evaluated the physico-chemical and biological properties of four different hyaluronated mesoporous silica nanoparticles (MSNs) samples (MSN/HA). Hyaluronic acid (HA) with two different molecular weights (200 and 6.4 kDa) was used for the conjugation of aminopropyl-functionalized MSN (NH2-MSN), following two different procedures. Namely, samples HA200A and HA6.4A were prepared by reacting activated HA with NH2-MSN (method A), while samples HA200B and HA6.4B were obtained carrying out HA activation in the presence of the nanoparticles (method B). The four samples showed similar hydrophilicity, but clear differences in the HA loading, textural properties, surface charge and stability of the suspensions. More in detail, conjugation using low molecular weight HA with method A resulted in low HA loading, with consequent scarce effects on dispersity and stability in physiological media. The highest yield and corresponding best performances were obtained with method B using high molecular weight HA. HA loading and molecular weight also influenced in a concerted way the biological response towards the MSNs of CD44 target cancer cells (CD44+) and control cells (CD44−): MDA-MB-231 and A2780, respectively. The absence of cytotoxicity was assessed. Moreover, the targeting ability of the best performing MSN/HA was confirmed by cellular uptake studies.
AB - We have prepared and evaluated the physico-chemical and biological properties of four different hyaluronated mesoporous silica nanoparticles (MSNs) samples (MSN/HA). Hyaluronic acid (HA) with two different molecular weights (200 and 6.4 kDa) was used for the conjugation of aminopropyl-functionalized MSN (NH2-MSN), following two different procedures. Namely, samples HA200A and HA6.4A were prepared by reacting activated HA with NH2-MSN (method A), while samples HA200B and HA6.4B were obtained carrying out HA activation in the presence of the nanoparticles (method B). The four samples showed similar hydrophilicity, but clear differences in the HA loading, textural properties, surface charge and stability of the suspensions. More in detail, conjugation using low molecular weight HA with method A resulted in low HA loading, with consequent scarce effects on dispersity and stability in physiological media. The highest yield and corresponding best performances were obtained with method B using high molecular weight HA. HA loading and molecular weight also influenced in a concerted way the biological response towards the MSNs of CD44 target cancer cells (CD44+) and control cells (CD44−): MDA-MB-231 and A2780, respectively. The absence of cytotoxicity was assessed. Moreover, the targeting ability of the best performing MSN/HA was confirmed by cellular uptake studies.
KW - Active targeting
KW - CD44 receptor
KW - Hyaluronic acid
KW - Hydrophilicity
KW - Infrared
KW - MSN
KW - Mesoporous silica nanoparticles
KW - Water adsorption
UR - http://www.scopus.com/inward/record.url?scp=85041466554&partnerID=8YFLogxK
U2 - 10.1016/j.jcis.2018.01.072
DO - 10.1016/j.jcis.2018.01.072
M3 - Article
SN - 0021-9797
VL - 516
SP - 484
EP - 497
JO - Journal of Colloid and Interface Science
JF - Journal of Colloid and Interface Science
ER -
