HPLC in the investigation of taxonomic problems. Classification of poplar genotypes

The RP-HPLC technique has been applied to the analysis of the flavonoid content of leaf extracts from various genotypes of Populus nigra and Populus deltoides and from several of their hybrids. Two elution procedures different either as regards gradient program or the type of organic modifier used were checked to optimize the reliability of the results obtained. Before making any identification, the chromatographic profiles proved so typical of each species as to allow of their use as fingerprints in this type of taxonomic problem. Individual identifications were carried out by spiking real samples with known amounts of standard substances. The presence of flavonoid compounds previously identified was confirmed and a few new identifications were proposed.