TY - JOUR
T1 - Histomorphometric, ultrastructural and microhardness evaluation of the osseointegration of a nanostructured titanium oxide coating by metal-organic chemical vapour deposition
T2 - An in vivo study
AU - Giavaresi, Gianluca
AU - Ambrosio, Luigi
AU - Battiston, Giovanni A.
AU - Casellato, Umberto
AU - Gerbasi, Rosalba
AU - Finia, Milena
AU - Aldini, Nicolò Nicoli
AU - Martini, Lucia
AU - Rimondini, Lia
AU - Giardino, Roberto
N1 - Funding Information:
The authors wish to acknowledge the Italian National Research Council: Progeti Finalizzati—Materiali Speciali per Tecnologie Avanzate II-Biomateriali, for the support of this research.
PY - 2004/11
Y1 - 2004/11
N2 - Over the past decade the increase of elderly population has determined a rise in the incidence of bone fractures, and the improvement of the implant-bone interface remains an open problem. Metal-organic chemical vapour deposition (MOCVD) has recently been proposed as a technique to coat orthopaedic and dental prostheses with metal nanostructured oxide films either through the decomposition of oxygenated compounds (single-source precursors) or the reaction of oxygen-free metal compounds with oxygenating agents. The present study was performed to assess the in vivo biocompatibility of commercially pure Ti (control material: TI/MA) implants (∅ 2mm×5mm length) coated with nanostructured TiO2 films by MOCVD (Ti/MOCVD) and then inserted into rabbit femoral cortical (middhiaphysis) and cancellous (distal epiphysis) bone. Histomorphometric, ultrastructural and microhardness investigations were carried out. Four and 12 weeks after surgery, significant (p<0.0005) increases in AI of Ti/MOCVD implants were observed as compared to Ti/MA implants (distal femoral epiphysis: 4 weeks=8.2%, ns; 12 weeks=52.3%, p<0.005; femoral diaphysis: 4 weeks=20.2%, p<0.0005; 12 weeks=10.7%, p<0.005). Bone microhardness results showed significant increases for the Ti/MOCVD versus Ti/MA implants at 200μm in the femoral diaphysis (4 weeks=14.2, p<0.005) and distal femoral epiphysis (12 weeks=14.5, p<0.01) at 4 and 12 weeks, respectively. In conclusion, the current findings demonstrate that the nanostructured TiO2 coating positively affects the osseointegration rate of commercially pure Ti implants and the bone mineralization at the bone-biomaterial interface in both cortical and cancellous bone.
AB - Over the past decade the increase of elderly population has determined a rise in the incidence of bone fractures, and the improvement of the implant-bone interface remains an open problem. Metal-organic chemical vapour deposition (MOCVD) has recently been proposed as a technique to coat orthopaedic and dental prostheses with metal nanostructured oxide films either through the decomposition of oxygenated compounds (single-source precursors) or the reaction of oxygen-free metal compounds with oxygenating agents. The present study was performed to assess the in vivo biocompatibility of commercially pure Ti (control material: TI/MA) implants (∅ 2mm×5mm length) coated with nanostructured TiO2 films by MOCVD (Ti/MOCVD) and then inserted into rabbit femoral cortical (middhiaphysis) and cancellous (distal epiphysis) bone. Histomorphometric, ultrastructural and microhardness investigations were carried out. Four and 12 weeks after surgery, significant (p<0.0005) increases in AI of Ti/MOCVD implants were observed as compared to Ti/MA implants (distal femoral epiphysis: 4 weeks=8.2%, ns; 12 weeks=52.3%, p<0.005; femoral diaphysis: 4 weeks=20.2%, p<0.0005; 12 weeks=10.7%, p<0.005). Bone microhardness results showed significant increases for the Ti/MOCVD versus Ti/MA implants at 200μm in the femoral diaphysis (4 weeks=14.2, p<0.005) and distal femoral epiphysis (12 weeks=14.5, p<0.01) at 4 and 12 weeks, respectively. In conclusion, the current findings demonstrate that the nanostructured TiO2 coating positively affects the osseointegration rate of commercially pure Ti implants and the bone mineralization at the bone-biomaterial interface in both cortical and cancellous bone.
KW - Histomorphometry
KW - Mechanical properties
KW - Metal surface treatment
KW - Osseointegration
KW - Scanning electron microscopy (SEM)
KW - Titanium oxide
UR - http://www.scopus.com/inward/record.url?scp=2542426844&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2004.01.017
DO - 10.1016/j.biomaterials.2004.01.017
M3 - Article
SN - 0142-9612
VL - 25
SP - 5583
EP - 5591
JO - Biomaterials
JF - Biomaterials
IS - 25
ER -