High-performance liquid chromatography-ultraviolet detection method for the simultaneous determination of typical biogenic amines and precursor amino acids. applications in food chemistry

Eleonora Mazzucco, Fabio Gosetti, Marco Bobba, Emilio Marengo, Elisa Robotti, Maria Carla Gennaro

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

A reversed-phase high-performance liquid chromatography (HPLC) method was developed for the simultaneous determination in food of biogenic amines and their precursor amino acids after a precolumn derivatization with dansyl chloride. The chromatographic conditions, selected to be suitable for mass spectrometry detection, were optimized through experimental design and artificial neural networks. The HPLC-UV method was validated by comparing the separation results with those obtained through a HPLC method, working under the same chromatographic conditions but employing mass spectrometry detection. The HPLC-UV method was then applied to the analysis of different food samples, namely, cheese, clams, salami, and beer. For all of the matrices, recoveries (relative standard deviation always <5%) always >92% were obtained. The results are discussed as a function of the total biogenic amine content and of the concentration ratio between amines and precursor amino acids.

Lingua originaleInglese
pagine (da-a)127-134
Numero di pagine8
RivistaJournal of Agricultural and Food Chemistry
Volume58
Numero di pubblicazione1
DOI
Stato di pubblicazionePubblicato - 13 gen 2010

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