TY - JOUR
T1 - High β-HPV DNA loads and strong seroreactivity are present in epidermodysplasia verruciformis
AU - Dell'Oste, Valentina
AU - Azzimonti, Barbara
AU - De Andrea, Marco
AU - Mondini, Michele
AU - Zavattaro, Elisa
AU - Leigheb, Giorgio
AU - Weissenborn, Sönke J.
AU - Pfister, Herbert
AU - Michael, Kristina M.
AU - Waterboer, Tim
AU - Pawlita, Michael
AU - Amantea, Ada
AU - Landolfo, Santo
AU - Gariglio, Marisa
N1 - Funding Information:
We thank M. Risio (IRCC, Turin) for providing tissue specimens from EV patient 3, and Ola Forslund (Lund University-Sweden) for providing plasmid clones for HPV types 93 and 96. This study was supported by “Bando Ricerca Applicata (CIPE 2004)”, and “Progetto Ricerca Sanitaria Finalizzata” 2006 and 2008 from Regione Piemonte. V.D.O. is a recipient of a PhD Fellowship from “Fondazione Cassa di Risparmio di Vercelli” and was also supported in part by Fondazione Cariplo. T.W was supported by the “Peter und Traudl Engelhorn-Stiftung zur Förderung der Biotechnologie und Gentechnik”, and S.W. by the “Deutsche Krebshilfe”.
PY - 2009/4
Y1 - 2009/4
N2 - Epidermodysplasia verruciformis (EV) is a rare disease, characterized by cutaneous warts and associated with a strong predisposition to β-genus human papillomavirus (HPV). Earlier studies reported high copy numbers of HPV-DNA in nearly all skin tumors from EV patients, but neither HPV replication status in non-lesional skin nor anti-HPV seroreactivity in these patients have been reported yet. We therefore performed a comprehensive viral load analysis for the more common β-HPV types on skin samples and plucked eyebrow hairs from four EV patients treated at our dermatology department. The results clearly demonstrate that they carry a multiplicity (up to eighteen types) of β-HPV genotypes in both skin sites. Worthy of note, a high intrapatient concordance for specific types between hair bulbs and skin biopsies was observed and the same β-PV profile was maintained over time. Viral load analysis revealed a load range between less than one HPV-DNA copy per 100 cells to more than 400 HPV-DNA copies per cell in both eyebrow hairs and skin proliferative lesions. Evaluation of seroreactivity to β-HPV types in the four EV patients revealed that antibodies against the 16 β-HPV were significantly more prevalent and showed higher titers than in the controls.
AB - Epidermodysplasia verruciformis (EV) is a rare disease, characterized by cutaneous warts and associated with a strong predisposition to β-genus human papillomavirus (HPV). Earlier studies reported high copy numbers of HPV-DNA in nearly all skin tumors from EV patients, but neither HPV replication status in non-lesional skin nor anti-HPV seroreactivity in these patients have been reported yet. We therefore performed a comprehensive viral load analysis for the more common β-HPV types on skin samples and plucked eyebrow hairs from four EV patients treated at our dermatology department. The results clearly demonstrate that they carry a multiplicity (up to eighteen types) of β-HPV genotypes in both skin sites. Worthy of note, a high intrapatient concordance for specific types between hair bulbs and skin biopsies was observed and the same β-PV profile was maintained over time. Viral load analysis revealed a load range between less than one HPV-DNA copy per 100 cells to more than 400 HPV-DNA copies per cell in both eyebrow hairs and skin proliferative lesions. Evaluation of seroreactivity to β-HPV types in the four EV patients revealed that antibodies against the 16 β-HPV were significantly more prevalent and showed higher titers than in the controls.
UR - https://www.scopus.com/pages/publications/63149132088
U2 - 10.1038/jid.2008.317
DO - 10.1038/jid.2008.317
M3 - Article
SN - 0022-202X
VL - 129
SP - 1026
EP - 1034
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 4
ER -
