Growth hormone releasing factor (GRF) increases free arachidonate levels in the pituitary: A role for lipoxygenase products

P. L. Canonico, C. Speciale, M. A. Sortino, M. J. Cronin, R. M. MacLeod, U. Scapagnini

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

GRF, a specific stimulator of GH release, increased in a concentration- and time-dependent manner pituitary [3H]- arachidonate levels in vitro. This effect was antagonized by 100 nM somatostatin. Exogenous arachidonate also stimulated GH release in vitro. Quinacrine, a phospholipase A2 inhibitor, reduced both basal and GRF-stimulated free arachidonate levels as well as GH release. The cyclooxygenase inhibitor indomethacin was ineffective, while BW755c, which also inhibits the lipoxygenase pathway, produced a further increase in the levels of the fatty acid stimulated by GRF and potently reduced GH release. These results provide additional evidence for the involvement of arachidonate metabolism in the hormone-releasing effect of GRF at the somatotroph.

Lingua originaleInglese
pagine (da-a)267-272
Numero di pagine6
RivistaLife Sciences
Volume38
Numero di pubblicazione3
DOI
Stato di pubblicazionePubblicato - 20 gen 1986
Pubblicato esternamente

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