Gene discovery in the tropical scallop Nodipecten nodosus: Construction and sequencing of a normalized cDNA library

Juliana Alves Americo; Francesco Dondero; Milton Ozório Moraes; Silvana Allodi; Mauro de Freitas Rebelo

doi:10.1016/j.marenvres.2013.03.008

Gene discovery in the tropical scallop Nodipecten nodosus: Construction and sequencing of a normalized cDNA library

Juliana Alves Americo
, Francesco Dondero
, Milton Ozório Moraes
, Silvana Allodi
, Mauro de Freitas Rebelo

Dipartimento di Scienze e Innovazione Tecnologica

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

We report the construction and characterization of a normalized cDNA library from the digestive gland of the marine bivalve Nodipecten nodosus, a commercially valuable tropical scallop. A total of 288 clones were sequenced, and 250 unique sequences were obtained. The cDNA library showed a small sequence redundancy (2.3%) and high numbers of recombinant (99.9%) and independent clones (2.0×10⁶cfu), indicating that the cDNA library generated in this study is a profitable resource for efficient gene discovery for N. nodosus. EST functional annotation by Gene Ontology term assignment revealed the identification of sequences potentially involved in aquaculture and ecotoxicology relevant processes such as apoptosis, growth, lipid metabolism, reproduction, development, response to stress and immunity.

Lingua originale	Inglese
pagine (da-a)	34-40
Numero di pagine	7
Rivista	Marine Environmental Research
Volume	91
DOI	https://doi.org/10.1016/j.marenvres.2013.03.008
Stato di pubblicazione	Pubblicato - ott 2013

OSS delle Nazioni Unite

Questo processo contribuisce al raggiungimento dei seguenti obiettivi di sviluppo sostenibile

SDG 14 La vita sott’acqua

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10.1016/j.marenvres.2013.03.008

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title = "Gene discovery in the tropical scallop Nodipecten nodosus: Construction and sequencing of a normalized cDNA library",

abstract = "We report the construction and characterization of a normalized cDNA library from the digestive gland of the marine bivalve Nodipecten nodosus, a commercially valuable tropical scallop. A total of 288 clones were sequenced, and 250 unique sequences were obtained. The cDNA library showed a small sequence redundancy (2.3\%) and high numbers of recombinant (99.9\%) and independent clones (2.0×106cfu), indicating that the cDNA library generated in this study is a profitable resource for efficient gene discovery for N. nodosus. EST functional annotation by Gene Ontology term assignment revealed the identification of sequences potentially involved in aquaculture and ecotoxicology relevant processes such as apoptosis, growth, lipid metabolism, reproduction, development, response to stress and immunity.",

keywords = "Aquaculture, EST, Lion's Paw scallop, MRNA, Stress",

author = "Americo, \{Juliana Alves\} and Francesco Dondero and Moraes, \{Milton Oz{\'o}rio\} and Silvana Allodi and Rebelo, \{Mauro de Freitas\}",

note = "Funding Information: This study was partially supported by FAPERJ ( E-26/111.540/2008 ). Dr. Rebelo is a member of the INCT-INPeTAm/CNPq/MCT, an Irving J. Selikoff Scholar of the Mount Sinai School of Medicine (award D43TW00640 from the Fogarty International Center), and a fellow of the Brazilian Research Council (CNPq 307401/2009-0). ",

year = "2013",

month = oct,

doi = "10.1016/j.marenvres.2013.03.008",

language = "English",

volume = "91",

pages = "34--40",

journal = "Marine Environmental Research",

issn = "0141-1136",

publisher = "Elsevier Ltd.",

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TY - JOUR

T1 - Gene discovery in the tropical scallop Nodipecten nodosus

T2 - Construction and sequencing of a normalized cDNA library

AU - Americo, Juliana Alves

AU - Dondero, Francesco

AU - Moraes, Milton Ozório

AU - Allodi, Silvana

AU - Rebelo, Mauro de Freitas

N1 - Funding Information: This study was partially supported by FAPERJ ( E-26/111.540/2008 ). Dr. Rebelo is a member of the INCT-INPeTAm/CNPq/MCT, an Irving J. Selikoff Scholar of the Mount Sinai School of Medicine (award D43TW00640 from the Fogarty International Center), and a fellow of the Brazilian Research Council (CNPq 307401/2009-0).

PY - 2013/10

Y1 - 2013/10

N2 - We report the construction and characterization of a normalized cDNA library from the digestive gland of the marine bivalve Nodipecten nodosus, a commercially valuable tropical scallop. A total of 288 clones were sequenced, and 250 unique sequences were obtained. The cDNA library showed a small sequence redundancy (2.3%) and high numbers of recombinant (99.9%) and independent clones (2.0×106cfu), indicating that the cDNA library generated in this study is a profitable resource for efficient gene discovery for N. nodosus. EST functional annotation by Gene Ontology term assignment revealed the identification of sequences potentially involved in aquaculture and ecotoxicology relevant processes such as apoptosis, growth, lipid metabolism, reproduction, development, response to stress and immunity.

AB - We report the construction and characterization of a normalized cDNA library from the digestive gland of the marine bivalve Nodipecten nodosus, a commercially valuable tropical scallop. A total of 288 clones were sequenced, and 250 unique sequences were obtained. The cDNA library showed a small sequence redundancy (2.3%) and high numbers of recombinant (99.9%) and independent clones (2.0×106cfu), indicating that the cDNA library generated in this study is a profitable resource for efficient gene discovery for N. nodosus. EST functional annotation by Gene Ontology term assignment revealed the identification of sequences potentially involved in aquaculture and ecotoxicology relevant processes such as apoptosis, growth, lipid metabolism, reproduction, development, response to stress and immunity.

KW - Aquaculture

KW - EST

KW - Lion's Paw scallop

KW - MRNA

KW - Stress

UR - https://www.scopus.com/pages/publications/84887167031

U2 - 10.1016/j.marenvres.2013.03.008

DO - 10.1016/j.marenvres.2013.03.008

M3 - Article

SN - 0141-1136

VL - 91

SP - 34

EP - 40

JO - Marine Environmental Research

JF - Marine Environmental Research

ER -

Gene discovery in the tropical scallop Nodipecten nodosus: Construction and sequencing of a normalized cDNA library

Abstract

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