TY - JOUR
T1 - Gene discovery in the tropical scallop Nodipecten nodosus
T2 - Construction and sequencing of a normalized cDNA library
AU - Americo, Juliana Alves
AU - Dondero, Francesco
AU - Moraes, Milton Ozório
AU - Allodi, Silvana
AU - Rebelo, Mauro de Freitas
N1 - Funding Information:
This study was partially supported by FAPERJ ( E-26/111.540/2008 ). Dr. Rebelo is a member of the INCT-INPeTAm/CNPq/MCT, an Irving J. Selikoff Scholar of the Mount Sinai School of Medicine (award D43TW00640 from the Fogarty International Center), and a fellow of the Brazilian Research Council (CNPq 307401/2009-0).
PY - 2013/10
Y1 - 2013/10
N2 - We report the construction and characterization of a normalized cDNA library from the digestive gland of the marine bivalve Nodipecten nodosus, a commercially valuable tropical scallop. A total of 288 clones were sequenced, and 250 unique sequences were obtained. The cDNA library showed a small sequence redundancy (2.3%) and high numbers of recombinant (99.9%) and independent clones (2.0×106cfu), indicating that the cDNA library generated in this study is a profitable resource for efficient gene discovery for N. nodosus. EST functional annotation by Gene Ontology term assignment revealed the identification of sequences potentially involved in aquaculture and ecotoxicology relevant processes such as apoptosis, growth, lipid metabolism, reproduction, development, response to stress and immunity.
AB - We report the construction and characterization of a normalized cDNA library from the digestive gland of the marine bivalve Nodipecten nodosus, a commercially valuable tropical scallop. A total of 288 clones were sequenced, and 250 unique sequences were obtained. The cDNA library showed a small sequence redundancy (2.3%) and high numbers of recombinant (99.9%) and independent clones (2.0×106cfu), indicating that the cDNA library generated in this study is a profitable resource for efficient gene discovery for N. nodosus. EST functional annotation by Gene Ontology term assignment revealed the identification of sequences potentially involved in aquaculture and ecotoxicology relevant processes such as apoptosis, growth, lipid metabolism, reproduction, development, response to stress and immunity.
KW - Aquaculture
KW - EST
KW - Lion's Paw scallop
KW - MRNA
KW - Stress
UR - https://www.scopus.com/pages/publications/84887167031
U2 - 10.1016/j.marenvres.2013.03.008
DO - 10.1016/j.marenvres.2013.03.008
M3 - Article
SN - 0141-1136
VL - 91
SP - 34
EP - 40
JO - Marine Environmental Research
JF - Marine Environmental Research
ER -
