GABP complex regulates transcription of eIF6 (p27BBP), an essential trans-acting factor in ribosome biogenesis

Alessandra Donadini; Francesca Giacopelli; Roberto Ravazzolo; Valentina Gandin; Pier Carlo Marchisio; Stefano Biffo

doi:10.1016/j.febslet.2006.02.068

GABP complex regulates transcription of eIF6 (p27^BBP), an essential trans-acting factor in ribosome biogenesis

Alessandra Donadini, Francesca Giacopelli, Roberto Ravazzolo, Valentina Gandin, Pier Carlo Marchisio, Stefano Biffo

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

Eukaryotic initiation factor 6 (eIF6, alias p27^BBP) is required for the biogenesis of 60S ribosomal subunits. eIF6 expression levels are tightly regulated in vivo, where they correlate with cellular growth. We analyzed how transcriptional regulation of eIF6 is achieved. We show that the human eIF6 promoter contains consensus sites for the GABP (GA-binding protein) transcription factor complex. Functional analysis of GABP consensus sequences by point mutations, EMSA (electrophoretic mobility shift assay) and a dominant negative mutant indicates that GABP is essential for eIF6 promoter activity. These data strengthen the hypothesis that GABP is a global regulator of ribosome synthesis.

Lingua originale	Inglese
pagine (da-a)	1983-1987
Numero di pagine	5
Rivista	FEBS Letters
Volume	580
Numero di pubblicazione	8
DOI	https://doi.org/10.1016/j.febslet.2006.02.068
Stato di pubblicazione	Pubblicato - 3 apr 2006
Pubblicato esternamente	Sì

Accesso al documento

10.1016/j.febslet.2006.02.068

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@article{cb47a3eacebb4945b0a026a25fdf37f8,

title = "GABP complex regulates transcription of eIF6 (p27BBP), an essential trans-acting factor in ribosome biogenesis",

abstract = "Eukaryotic initiation factor 6 (eIF6, alias p27BBP) is required for the biogenesis of 60S ribosomal subunits. eIF6 expression levels are tightly regulated in vivo, where they correlate with cellular growth. We analyzed how transcriptional regulation of eIF6 is achieved. We show that the human eIF6 promoter contains consensus sites for the GABP (GA-binding protein) transcription factor complex. Functional analysis of GABP consensus sequences by point mutations, EMSA (electrophoretic mobility shift assay) and a dominant negative mutant indicates that GABP is essential for eIF6 promoter activity. These data strengthen the hypothesis that GABP is a global regulator of ribosome synthesis.",

keywords = "Beta binding protein (p27BBP), ITGB4PB gene, Nucleolus, Translation",

author = "Alessandra Donadini and Francesca Giacopelli and Roberto Ravazzolo and Valentina Gandin and Marchisio, \{Pier Carlo\} and Stefano Biffo",

note = "Funding Information: This work is supported from AIRC grant to S.B. and P.C.M. We thank M. Sunesen and Professor J.P. Changeux for dnGABP, and O. Baldi for technical help. We thank W. Leonard and members of his lab for their double attempt to provide us with MEFs mutants for GABP. ",

year = "2006",

month = apr,

day = "3",

doi = "10.1016/j.febslet.2006.02.068",

language = "English",

volume = "580",

pages = "1983--1987",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "8",

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TY - JOUR

T1 - GABP complex regulates transcription of eIF6 (p27BBP), an essential trans-acting factor in ribosome biogenesis

AU - Donadini, Alessandra

AU - Giacopelli, Francesca

AU - Ravazzolo, Roberto

AU - Gandin, Valentina

AU - Marchisio, Pier Carlo

AU - Biffo, Stefano

N1 - Funding Information: This work is supported from AIRC grant to S.B. and P.C.M. We thank M. Sunesen and Professor J.P. Changeux for dnGABP, and O. Baldi for technical help. We thank W. Leonard and members of his lab for their double attempt to provide us with MEFs mutants for GABP.

PY - 2006/4/3

Y1 - 2006/4/3

N2 - Eukaryotic initiation factor 6 (eIF6, alias p27BBP) is required for the biogenesis of 60S ribosomal subunits. eIF6 expression levels are tightly regulated in vivo, where they correlate with cellular growth. We analyzed how transcriptional regulation of eIF6 is achieved. We show that the human eIF6 promoter contains consensus sites for the GABP (GA-binding protein) transcription factor complex. Functional analysis of GABP consensus sequences by point mutations, EMSA (electrophoretic mobility shift assay) and a dominant negative mutant indicates that GABP is essential for eIF6 promoter activity. These data strengthen the hypothesis that GABP is a global regulator of ribosome synthesis.

AB - Eukaryotic initiation factor 6 (eIF6, alias p27BBP) is required for the biogenesis of 60S ribosomal subunits. eIF6 expression levels are tightly regulated in vivo, where they correlate with cellular growth. We analyzed how transcriptional regulation of eIF6 is achieved. We show that the human eIF6 promoter contains consensus sites for the GABP (GA-binding protein) transcription factor complex. Functional analysis of GABP consensus sequences by point mutations, EMSA (electrophoretic mobility shift assay) and a dominant negative mutant indicates that GABP is essential for eIF6 promoter activity. These data strengthen the hypothesis that GABP is a global regulator of ribosome synthesis.

KW - Beta binding protein (p27BBP)

KW - ITGB4PB gene

KW - Nucleolus

KW - Translation

UR - https://www.scopus.com/pages/publications/33645229675

U2 - 10.1016/j.febslet.2006.02.068

DO - 10.1016/j.febslet.2006.02.068

M3 - Article

SN - 0014-5793

VL - 580

SP - 1983

EP - 1987

JO - FEBS Letters

JF - FEBS Letters

IS - 8