TY - JOUR
T1 - Formulation of microspheres containing Crataegus monogyna Jacq. extract with free radical scavenging activity
AU - Lucconi, Giulia
AU - Chlapanidas, Theodora
AU - Martino, Emanuela
AU - Gaggeri, Raffaella
AU - Perteghella, Sara
AU - Rossi, Daniela
AU - Faragò, Silvio
AU - Vigo, Daniele
AU - Faustini, Massimo
AU - Collina, Simona
AU - Torre, Maria Luisa
PY - 2014
Y1 - 2014
N2 - Extracts of Crataegus monogyna Jacq. (hawthorn) show an interesting free radical scavenging (FRS) effect, related to their flavonoids content. Unfortunately, their oral administration is affected by their low bioavailability. The aim of this work is to obtain a multiparticulate drug delivery system for hawthorn extracts for oral administration. The extracts from flowering tops (FL) or fruits (FR) of hawthorn were obtained with maceration, using ethanol as an extraction solvent, and their antioxidant activity was evaluated. FL extract showed the highest FRS activity (EC50 3.72 ± 1.21 μg/ml), so it was selected to prepare microparticulate systems by a spraydrying technique, which were characterized by granulometric analysis, scanning electron microscopy-energy dispersive X-ray spectroscopy, confocal fluorescence microscopy and hyperoside content. Antioxidant activity was evaluated before and after gastrointestinal transit in vitro simulation. Results indicate that the microparticulate systems maintained the antioxidant activity of hawthorn also after gastrointestinal transit in vitro simulation, exhibiting properties suitable for oral administration.
AB - Extracts of Crataegus monogyna Jacq. (hawthorn) show an interesting free radical scavenging (FRS) effect, related to their flavonoids content. Unfortunately, their oral administration is affected by their low bioavailability. The aim of this work is to obtain a multiparticulate drug delivery system for hawthorn extracts for oral administration. The extracts from flowering tops (FL) or fruits (FR) of hawthorn were obtained with maceration, using ethanol as an extraction solvent, and their antioxidant activity was evaluated. FL extract showed the highest FRS activity (EC50 3.72 ± 1.21 μg/ml), so it was selected to prepare microparticulate systems by a spraydrying technique, which were characterized by granulometric analysis, scanning electron microscopy-energy dispersive X-ray spectroscopy, confocal fluorescence microscopy and hyperoside content. Antioxidant activity was evaluated before and after gastrointestinal transit in vitro simulation. Results indicate that the microparticulate systems maintained the antioxidant activity of hawthorn also after gastrointestinal transit in vitro simulation, exhibiting properties suitable for oral administration.
KW - Crataegus monogyna Jacq.
KW - Free radical scavenging activity
KW - Hawthorn
KW - Microcrosphere
UR - http://www.scopus.com/inward/record.url?scp=84899993963&partnerID=8YFLogxK
U2 - 10.3109/10837450.2012.752387
DO - 10.3109/10837450.2012.752387
M3 - Article
SN - 1083-7450
VL - 19
SP - 65
EP - 72
JO - Pharmaceutical Development and Technology
JF - Pharmaceutical Development and Technology
IS - 1
ER -