Follicle-like model by granulosa cell encapsulation in a barium alginate-protamine membrane

D VIGO; SIMONA VILLANI; M FAUSTINI; PA ACCORSI; G GALEATI; M SPINACI; E MUNARI; V RUSSO; A ASTI; UBALDO CONTE; MARIA LUISA TORRE

doi:10.1089/ten.2005.11.709

Follicle-like model by granulosa cell encapsulation in a barium alginate-protamine membrane

D VIGO, SIMONA VILLANI, M FAUSTINI, PA ACCORSI, G GALEATI, M SPINACI, E MUNARI, V RUSSO, A ASTI, UBALDO CONTE, MARIA LUISA TORRE

Dipartimento di Scienze del Farmaco

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-like barium alginate capsules for 6 days. Morphological investigation by electron scanning microscopy indicated that culture in a three-dimensional (3D) system allows self-organization of spherical-polyhedral shape cells. The luteinization index (progesterone:17beta-estradiol ratio) was significantly higher for monolayer cells than for the 3D cell culture system, confirming the results of morphological analysis and indicating more physiological growth. The encapsulated 3D culture system appears to be a promising way of obtaining in vitro maturation and development of follicles and oocytes.

Lingua originale	Inglese
pagine (da-a)	709-714
Numero di pagine	6
Rivista	Tissue Engineering
Volume	11
Numero di pubblicazione	5/6
DOI	https://doi.org/10.1089/ten.2005.11.709
Stato di pubblicazione	Pubblicato - 1 gen 2005

Keywords

3D granulosa cells culture
capsules
in vitro maturation

Accesso al documento

10.1089/ten.2005.11.709

http://hdl.handle.net/11579/144465

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title = "Follicle-like model by granulosa cell encapsulation in a barium alginate-protamine membrane",

abstract = "Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-like barium alginate capsules for 6 days. Morphological investigation by electron scanning microscopy indicated that culture in a three-dimensional (3D) system allows self-organization of spherical-polyhedral shape cells. The luteinization index (progesterone:17beta-estradiol ratio) was significantly higher for monolayer cells than for the 3D cell culture system, confirming the results of morphological analysis and indicating more physiological growth. The encapsulated 3D culture system appears to be a promising way of obtaining in vitro maturation and development of follicles and oocytes.",

keywords = "3D granulosa cells culture, capsules, in vitro maturation, 3D granulosa cells culture, capsules, in vitro maturation",

author = "D VIGO and SIMONA VILLANI and M FAUSTINI and PA ACCORSI and G GALEATI and M SPINACI and E MUNARI and V RUSSO and A ASTI and UBALDO CONTE and TORRE, {MARIA LUISA}",

year = "2005",

month = jan,

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doi = "10.1089/ten.2005.11.709",

language = "English",

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journal = "Tissue Engineering",

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T1 - Follicle-like model by granulosa cell encapsulation in a barium alginate-protamine membrane

AU - VIGO, D

AU - VILLANI, SIMONA

AU - FAUSTINI, M

AU - ACCORSI, PA

AU - GALEATI, G

AU - SPINACI, M

AU - MUNARI, E

AU - RUSSO, V

AU - ASTI, A

AU - CONTE, UBALDO

AU - TORRE, MARIA LUISA

PY - 2005/1/1

Y1 - 2005/1/1

N2 - Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-like barium alginate capsules for 6 days. Morphological investigation by electron scanning microscopy indicated that culture in a three-dimensional (3D) system allows self-organization of spherical-polyhedral shape cells. The luteinization index (progesterone:17beta-estradiol ratio) was significantly higher for monolayer cells than for the 3D cell culture system, confirming the results of morphological analysis and indicating more physiological growth. The encapsulated 3D culture system appears to be a promising way of obtaining in vitro maturation and development of follicles and oocytes.

AB - Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-like barium alginate capsules for 6 days. Morphological investigation by electron scanning microscopy indicated that culture in a three-dimensional (3D) system allows self-organization of spherical-polyhedral shape cells. The luteinization index (progesterone:17beta-estradiol ratio) was significantly higher for monolayer cells than for the 3D cell culture system, confirming the results of morphological analysis and indicating more physiological growth. The encapsulated 3D culture system appears to be a promising way of obtaining in vitro maturation and development of follicles and oocytes.

KW - 3D granulosa cells culture

KW - capsules

KW - in vitro maturation

KW - 3D granulosa cells culture

KW - capsules

KW - in vitro maturation

UR - https://iris.uniupo.it/handle/11579/144465

U2 - 10.1089/ten.2005.11.709

DO - 10.1089/ten.2005.11.709

M3 - Article

SN - 1076-3279

VL - 11

SP - 709

EP - 714

JO - Tissue Engineering

JF - Tissue Engineering

IS - 5/6

ER -

Follicle-like model by granulosa cell encapsulation in a barium alginate-protamine membrane

Abstract

Keywords

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