Fibroblast apoptosis and caspase-8 activation in aseptic loosening

F. Renò, M. Sabbatini, A. Massè, M. Bosetti, M. Cannas

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

The presence of apoptosis has been investigated in the interface membranes collected during revision surgery of loosened total hip joint arthroplasty (THAs). Terminal deoxyrobonucleotidyl transferase (TdT) assay for apoptotic DNA fragmentation quantification revealed a statistically significant presence of apoptosis in aseptic samples, obtained from both cementless (2.37±0.6%) and cemented (12.01±1%) prosthesis compared to septic samples where apoptosis was almost absent. Activated caspase-8 immunostaining was almost undetectable in septic samples, while in the aseptic samples active caspase-8 was present weakly in the cementless samples (1.35±0.22%) and strongly in the cemented ones (9.0±0.40%). The caspase-8 cytoplasmatic staining allowed the morphological recognition of positive cells both as fibroblast-like and immunocompetent cells. In aseptic cemented samples fibroblast-like cells were the most represented subpopulation in the caspase-8 positive population scored (76.6%) compared to the immunocompetent cells (23.4%). Caspase-8 activation is an upstream event in the apoptotic pathway triggered by the activation of cytokines receptors such as TNF-α receptor 1 (TNFR-1), and the presence of caspase-8 activation in fibroblast-like cells in the aseptic interface membranes of THAs suggests a possible TNF-α dependent apoptosis.

Lingua originaleInglese
pagine (da-a)3941-3946
Numero di pagine6
RivistaBiomaterials
Volume24
Numero di pubblicazione22
DOI
Stato di pubblicazionePubblicato - ott 2003

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