Efficient Route to Label Mesenchymal Stromal Cell-Derived Extracellular Vesicles

Diego Alberti, Cristina Grange, Stefano Porta, Silvio Aime, Lorenzo Tei, Simonetta Geninatti Crich

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

Recent research results report that extracellular vesicles (EVs) have a central role in both physiological and pathological processes involving intercellular communication. Herein, a simple EVs labeling procedure based on the metabolic labeling of secreting cells (mesenchymal stroma cells, MSCs) with a fluorescein-containing bio-orthogonal dye is described. This procedure was carried out by incubating cells for 72 h with tetraacetylated N-azidoacetyl-d-mannosamine (Ac4ManNAz), a modified sugar containing an azido group that, upon incorporation on the external surface of the cytoplasmatic cell membrane, is specifically conjugated with cyclooctyne-modified fluorescein isothiocyanate (ADIBO-FITC). MSCs released fluorescent EVs did not need any further purification. Finally, cellular uptake and tracking of the fluorescein-labeled EVs were successfully assessed by targeting experiments with MSCs. The method appears of general applicability and it may be very useful opening new horizon on diagnostic and therapeutic protocols exploiting EVs.

Lingua originaleInglese
pagine (da-a)8097-8103
Numero di pagine7
RivistaACS Omega
Volume3
Numero di pubblicazione7
DOI
Stato di pubblicazionePubblicato - 31 lug 2018

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