TY - JOUR
T1 - Effects of the human cd38 glycoprotein on the early stages of the HIV-1 replication cycle
AU - Savarino, Andrea
AU - Bottarel, Flavia
AU - Calosso, Liliana
AU - Feito, Maria Josè
AU - Bensi, Thea
AU - Bragardo, Manuela
AU - Rojo, Josè Maria
AU - Pugliese, Agostino
AU - Abbate, Isabella
AU - Capobianchi, Maria R.
AU - Dianzani, Ferdinando
AU - Malavasi, Fabio
AU - Dianzani, Umberto
PY - 1999
Y1 - 1999
N2 - CD38 displays lateral association with the HIV-1 receptor CD4. This association is potentiated by the HIV-1 envelope glycoprotein gp120. The aim of this work was to evaluate the CD38 role in T cell susceptibility to HIV-1 infection. Using laboratory X4 HIV-1 strains and X4 and X4/R5 primary isolates, we found that CD38 expression was negatively correlated to cell susceptibility to infection, evaluated as percentage of infected cells, release of HIV p24 in the supernatants, and cytopathogenicity. This correlation was at first suggested by results obtained in a panel of human CD4+ T cell lines expressing different CD38 levels (MT-4, MT-2, C8166, CEMx174, Supt-1, and H9) and then demonstrated using CD38 transfectants of MT-4 cells (the line with the lowest CD38 expression). To address whether CD38 affected viral binding, we used mouse T cells that are non-permissive for productive infection. Gene transfection in mouse SR.D10.CD4-.F1 T cells produced four lines expressing human CD4 and/or CD38. Ability of CD4+CD38+ cells to bind HIV-1 or purified recombinant gp120 was significantly lower than that of CD4+CD38- cells. These data suggest that CD38 expression inhibits lymphocyte susceptibility to HIV infection, probably by inhibiting gp120/CD4-dependent viral binding to target cells. - Savarino, A., Bottarel, F., Calosso, L., Feito, M. J., Bensi, T., Bragardo, M., Rojo, J. M., Pugliese, A., Abbate, I., Capobianchi, M. R., Dianzani, F., Malavasi, F., and Dianzani, U. Effects of the human CD38 glycoprotein on the early stages of the HIV-1 replication cycle.
AB - CD38 displays lateral association with the HIV-1 receptor CD4. This association is potentiated by the HIV-1 envelope glycoprotein gp120. The aim of this work was to evaluate the CD38 role in T cell susceptibility to HIV-1 infection. Using laboratory X4 HIV-1 strains and X4 and X4/R5 primary isolates, we found that CD38 expression was negatively correlated to cell susceptibility to infection, evaluated as percentage of infected cells, release of HIV p24 in the supernatants, and cytopathogenicity. This correlation was at first suggested by results obtained in a panel of human CD4+ T cell lines expressing different CD38 levels (MT-4, MT-2, C8166, CEMx174, Supt-1, and H9) and then demonstrated using CD38 transfectants of MT-4 cells (the line with the lowest CD38 expression). To address whether CD38 affected viral binding, we used mouse T cells that are non-permissive for productive infection. Gene transfection in mouse SR.D10.CD4-.F1 T cells produced four lines expressing human CD4 and/or CD38. Ability of CD4+CD38+ cells to bind HIV-1 or purified recombinant gp120 was significantly lower than that of CD4+CD38- cells. These data suggest that CD38 expression inhibits lymphocyte susceptibility to HIV infection, probably by inhibiting gp120/CD4-dependent viral binding to target cells. - Savarino, A., Bottarel, F., Calosso, L., Feito, M. J., Bensi, T., Bragardo, M., Rojo, J. M., Pugliese, A., Abbate, I., Capobianchi, M. R., Dianzani, F., Malavasi, F., and Dianzani, U. Effects of the human CD38 glycoprotein on the early stages of the HIV-1 replication cycle.
KW - Susceptibility to HIV-1
KW - gp120/CD4-dependent virus binding
UR - http://www.scopus.com/inward/record.url?scp=0032787504&partnerID=8YFLogxK
U2 - 10.1096/fasebj.13.15.2265
DO - 10.1096/fasebj.13.15.2265
M3 - Article
SN - 0892-6638
VL - 13
SP - 2265
EP - 2276
JO - FASEB Journal
JF - FASEB Journal
IS - 15
ER -