Effects of sodium and potassium adenosine-triphosphatase on circulating lymphocytes: An approach to human obesity

Chiarella Bozzo; Mariella Goria; Saverio Marena; Vittorio Tagliaferro; Gianfranco Pagano

doi:10.1007/BF02886839

Effects of sodium and potassium adenosine-triphosphatase on circulating lymphocytes: An approach to human obesity

Chiarella Bozzo
, Mariella Goria
, Saverio Marena
, Vittorio Tagliaferro
, Gianfranco Pagano

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

The methodological aspects of (Na⁺,K⁺)-ATPase-dependent uptake of⁸⁶Rb, a potassium analog, were examined on human lymphocytes isolated from peripheral blood. The study of the time-course, the kinetic parameters, i.e., maximum velocity (V_max) and Michaelis constant (K_m) and the ouabain inhibition curve of⁸⁶Rb⁺ uptake confirm that circulating lymphocytes represent a suitable model for the study of (Na⁺,K⁺)-ATPase in human diseases. An application to human obesity is reported: the results indicate that⁸⁶Rb⁺ uptake on circulating lymphocytes is similar in obese and non-obese subjects. Therefore, (Na⁺,K⁺)-ATPase does not seem to be involved in the pathogenesis of human obesity.

Lingua originale	Inglese
pagine (da-a)	555-561
Numero di pagine	7
Rivista	La Ricerca in Clinica e in Laboratorio
Volume	16
Numero di pubblicazione	4
DOI	https://doi.org/10.1007/BF02886839
Stato di pubblicazione	Pubblicato - ott 1986
Pubblicato esternamente	Sì

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title = "Effects of sodium and potassium adenosine-triphosphatase on circulating lymphocytes: An approach to human obesity",

abstract = "The methodological aspects of (Na+,K+)-ATPase-dependent uptake of86Rb, a potassium analog, were examined on human lymphocytes isolated from peripheral blood. The study of the time-course, the kinetic parameters, i.e., maximum velocity (Vmax) and Michaelis constant (Km) and the ouabain inhibition curve of86Rb+ uptake confirm that circulating lymphocytes represent a suitable model for the study of (Na+,K+)-ATPase in human diseases. An application to human obesity is reported: the results indicate that86Rb+ uptake on circulating lymphocytes is similar in obese and non-obese subjects. Therefore, (Na+,K+)-ATPase does not seem to be involved in the pathogenesis of human obesity.",

keywords = "Circulating lymphocytes, Human obesity, Ouabain, Rubidium uptake, Sodium and potassium adenosine-triphosphatase, Sodium-potassium pump",

author = "Chiarella Bozzo and Mariella Goria and Saverio Marena and Vittorio Tagliaferro and Gianfranco Pagano",

year = "1986",

month = oct,

doi = "10.1007/BF02886839",

language = "English",

volume = "16",

pages = "555--561",

journal = "La Ricerca in Clinica e in Laboratorio",

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}

TY - JOUR

T1 - Effects of sodium and potassium adenosine-triphosphatase on circulating lymphocytes

T2 - An approach to human obesity

AU - Bozzo, Chiarella

AU - Goria, Mariella

AU - Marena, Saverio

AU - Tagliaferro, Vittorio

AU - Pagano, Gianfranco

PY - 1986/10

Y1 - 1986/10

N2 - The methodological aspects of (Na+,K+)-ATPase-dependent uptake of86Rb, a potassium analog, were examined on human lymphocytes isolated from peripheral blood. The study of the time-course, the kinetic parameters, i.e., maximum velocity (Vmax) and Michaelis constant (Km) and the ouabain inhibition curve of86Rb+ uptake confirm that circulating lymphocytes represent a suitable model for the study of (Na+,K+)-ATPase in human diseases. An application to human obesity is reported: the results indicate that86Rb+ uptake on circulating lymphocytes is similar in obese and non-obese subjects. Therefore, (Na+,K+)-ATPase does not seem to be involved in the pathogenesis of human obesity.

AB - The methodological aspects of (Na+,K+)-ATPase-dependent uptake of86Rb, a potassium analog, were examined on human lymphocytes isolated from peripheral blood. The study of the time-course, the kinetic parameters, i.e., maximum velocity (Vmax) and Michaelis constant (Km) and the ouabain inhibition curve of86Rb+ uptake confirm that circulating lymphocytes represent a suitable model for the study of (Na+,K+)-ATPase in human diseases. An application to human obesity is reported: the results indicate that86Rb+ uptake on circulating lymphocytes is similar in obese and non-obese subjects. Therefore, (Na+,K+)-ATPase does not seem to be involved in the pathogenesis of human obesity.

KW - Circulating lymphocytes

KW - Human obesity

KW - Ouabain

KW - Rubidium uptake

KW - Sodium and potassium adenosine-triphosphatase

KW - Sodium-potassium pump

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U2 - 10.1007/BF02886839

DO - 10.1007/BF02886839

M3 - Article

SN - 0390-5748

VL - 16

SP - 555

EP - 561

JO - La Ricerca in Clinica e in Laboratorio

JF - La Ricerca in Clinica e in Laboratorio

IS - 4

ER -

Effects of sodium and potassium adenosine-triphosphatase on circulating lymphocytes: An approach to human obesity

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