Effects of sodium and potassium adenosine-triphosphatase on circulating lymphocytes: An approach to human obesity

Chiarella Bozzo, Mariella Goria, Saverio Marena, Vittorio Tagliaferro, Gianfranco Pagano

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

The methodological aspects of (Na+,K+)-ATPase-dependent uptake of86Rb, a potassium analog, were examined on human lymphocytes isolated from peripheral blood. The study of the time-course, the kinetic parameters, i.e., maximum velocity (Vmax) and Michaelis constant (Km) and the ouabain inhibition curve of86Rb+ uptake confirm that circulating lymphocytes represent a suitable model for the study of (Na+,K+)-ATPase in human diseases. An application to human obesity is reported: the results indicate that86Rb+ uptake on circulating lymphocytes is similar in obese and non-obese subjects. Therefore, (Na+,K+)-ATPase does not seem to be involved in the pathogenesis of human obesity.

Lingua originaleInglese
pagine (da-a)555-561
Numero di pagine7
RivistaLa Ricerca in Clinica e in Laboratorio
Volume16
Numero di pubblicazione4
DOI
Stato di pubblicazionePubblicato - ott 1986
Pubblicato esternamente

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