TY - JOUR
T1 - Effects of sodium and potassium adenosine-triphosphatase on circulating lymphocytes
T2 - An approach to human obesity
AU - Bozzo, Chiarella
AU - Goria, Mariella
AU - Marena, Saverio
AU - Tagliaferro, Vittorio
AU - Pagano, Gianfranco
PY - 1986/10
Y1 - 1986/10
N2 - The methodological aspects of (Na+,K+)-ATPase-dependent uptake of86Rb, a potassium analog, were examined on human lymphocytes isolated from peripheral blood. The study of the time-course, the kinetic parameters, i.e., maximum velocity (Vmax) and Michaelis constant (Km) and the ouabain inhibition curve of86Rb+ uptake confirm that circulating lymphocytes represent a suitable model for the study of (Na+,K+)-ATPase in human diseases. An application to human obesity is reported: the results indicate that86Rb+ uptake on circulating lymphocytes is similar in obese and non-obese subjects. Therefore, (Na+,K+)-ATPase does not seem to be involved in the pathogenesis of human obesity.
AB - The methodological aspects of (Na+,K+)-ATPase-dependent uptake of86Rb, a potassium analog, were examined on human lymphocytes isolated from peripheral blood. The study of the time-course, the kinetic parameters, i.e., maximum velocity (Vmax) and Michaelis constant (Km) and the ouabain inhibition curve of86Rb+ uptake confirm that circulating lymphocytes represent a suitable model for the study of (Na+,K+)-ATPase in human diseases. An application to human obesity is reported: the results indicate that86Rb+ uptake on circulating lymphocytes is similar in obese and non-obese subjects. Therefore, (Na+,K+)-ATPase does not seem to be involved in the pathogenesis of human obesity.
KW - Circulating lymphocytes
KW - Human obesity
KW - Ouabain
KW - Rubidium uptake
KW - Sodium and potassium adenosine-triphosphatase
KW - Sodium-potassium pump
UR - http://www.scopus.com/inward/record.url?scp=0022789550&partnerID=8YFLogxK
U2 - 10.1007/BF02886839
DO - 10.1007/BF02886839
M3 - Article
SN - 0390-5748
VL - 16
SP - 555
EP - 561
JO - La Ricerca in Clinica e in Laboratorio
JF - La Ricerca in Clinica e in Laboratorio
IS - 4
ER -