Effects of IFI16 overexpression on the growth and doxorubicin sensitivity of head and neck squamous cell carcinoma-derived cell lines

Marco De Andrea; Daniela Gioia; Michele Mondini; Barbara Azzimonti; Filippo Renò; Giancarlo Pecorari; Vincenzo Landolfo; Massimo Tommasino; Rosita Accardi; Christel Herold-Mende; Santo Landolfo; Marisa Gariglio

doi:10.1002/hed.20611

Effects of IFI16 overexpression on the growth and doxorubicin sensitivity of head and neck squamous cell carcinoma-derived cell lines

Marco De Andrea, Daniela Gioia, Michele Mondini, Barbara Azzimonti, Filippo Renò, Giancarlo Pecorari, Vincenzo Landolfo, Massimo Tommasino, Rosita Accardi, Christel Herold-Mende, Santo Landolfo, Marisa Gariglio

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

Background. In a previous analysis of head and neck squamous cell carcinomas (HNSCCs), we showed that the levels of the interferon-inducible protein IFI16 inversely correlate with cancer grade. In this study, we further evaluate the molecular role of IFI16 in the development of HNSCCs. Methods. The effect of IFI16 expression was evaluated by its retroviral restoration in an IFI16-negative HNSCC-derived cell line, HNO136. Growth rate and soft agar colony formation were evaluated. The effect of IFI16 restoration in cells exposed to doxorubicin was also analyzed. Results. IFI16 restoration resulted in the inhibition of both cell growth and in vitro transforming activity and increased doxorubicin-induced cell death by accumulating the cells at the G2/M phase. Conclusion. In agreement with our previous in vivo data, IFI16 appears to be involved in maintaining the normal growth of epithelial cells, whereas its downregulation may contribute to uncontrolled cell proliferation and tumorigenesis.

Lingua originale	Inglese
pagine (da-a)	835-844
Numero di pagine	10
Rivista	Head and Neck
Volume	29
Numero di pubblicazione	9
DOI	https://doi.org/10.1002/hed.20611
Stato di pubblicazione	Pubblicato - set 2007
Pubblicato esternamente	Sì

OSS delle Nazioni Unite

Questo processo contribuisce al raggiungimento dei seguenti obiettivi di sviluppo sostenibile

Accesso al documento

10.1002/hed.20611

Altri file e collegamenti

Link to publication in Scopus

Cita questo

De Andrea, M., Gioia, D., Mondini, M., Azzimonti, B., Renò, F., Pecorari, G., Landolfo, V., Tommasino, M., Accardi, R., Herold-Mende, C., Landolfo, S., & Gariglio, M. (2007). Effects of IFI16 overexpression on the growth and doxorubicin sensitivity of head and neck squamous cell carcinoma-derived cell lines. Head and Neck, 29(9), 835-844. https://doi.org/10.1002/hed.20611

@article{3faa122c4598402bad2e30f8fbd4d785,

title = "Effects of IFI16 overexpression on the growth and doxorubicin sensitivity of head and neck squamous cell carcinoma-derived cell lines",

abstract = "Background. In a previous analysis of head and neck squamous cell carcinomas (HNSCCs), we showed that the levels of the interferon-inducible protein IFI16 inversely correlate with cancer grade. In this study, we further evaluate the molecular role of IFI16 in the development of HNSCCs. Methods. The effect of IFI16 expression was evaluated by its retroviral restoration in an IFI16-negative HNSCC-derived cell line, HNO136. Growth rate and soft agar colony formation were evaluated. The effect of IFI16 restoration in cells exposed to doxorubicin was also analyzed. Results. IFI16 restoration resulted in the inhibition of both cell growth and in vitro transforming activity and increased doxorubicin-induced cell death by accumulating the cells at the G2/M phase. Conclusion. In agreement with our previous in vivo data, IFI16 appears to be involved in maintaining the normal growth of epithelial cells, whereas its downregulation may contribute to uncontrolled cell proliferation and tumorigenesis.",

keywords = "Doxorubicin, Growth arrest, Head and neck squamous cell carcinoma, IFI16, Interferon",

author = "{De Andrea}, Marco and Daniela Gioia and Michele Mondini and Barbara Azzimonti and Filippo Ren{\`o} and Giancarlo Pecorari and Vincenzo Landolfo and Massimo Tommasino and Rosita Accardi and Christel Herold-Mende and Santo Landolfo and Marisa Gariglio",

year = "2007",

month = sep,

doi = "10.1002/hed.20611",

language = "English",

volume = "29",

pages = "835--844",

journal = "Head and Neck",

issn = "1043-3074",

publisher = "John Wiley & Sons Inc.",

number = "9",

}

De Andrea, M, Gioia, D, Mondini, M, Azzimonti, B, Renò, F, Pecorari, G, Landolfo, V, Tommasino, M, Accardi, R, Herold-Mende, C, Landolfo, S & Gariglio, M 2007, 'Effects of IFI16 overexpression on the growth and doxorubicin sensitivity of head and neck squamous cell carcinoma-derived cell lines', Head and Neck, vol. 29, n. 9, pagg. 835-844. https://doi.org/10.1002/hed.20611

TY - JOUR

T1 - Effects of IFI16 overexpression on the growth and doxorubicin sensitivity of head and neck squamous cell carcinoma-derived cell lines

AU - De Andrea, Marco

AU - Gioia, Daniela

AU - Mondini, Michele

AU - Azzimonti, Barbara

AU - Renò, Filippo

AU - Pecorari, Giancarlo

AU - Landolfo, Vincenzo

AU - Tommasino, Massimo

AU - Accardi, Rosita

AU - Herold-Mende, Christel

AU - Landolfo, Santo

AU - Gariglio, Marisa

PY - 2007/9

Y1 - 2007/9

N2 - Background. In a previous analysis of head and neck squamous cell carcinomas (HNSCCs), we showed that the levels of the interferon-inducible protein IFI16 inversely correlate with cancer grade. In this study, we further evaluate the molecular role of IFI16 in the development of HNSCCs. Methods. The effect of IFI16 expression was evaluated by its retroviral restoration in an IFI16-negative HNSCC-derived cell line, HNO136. Growth rate and soft agar colony formation were evaluated. The effect of IFI16 restoration in cells exposed to doxorubicin was also analyzed. Results. IFI16 restoration resulted in the inhibition of both cell growth and in vitro transforming activity and increased doxorubicin-induced cell death by accumulating the cells at the G2/M phase. Conclusion. In agreement with our previous in vivo data, IFI16 appears to be involved in maintaining the normal growth of epithelial cells, whereas its downregulation may contribute to uncontrolled cell proliferation and tumorigenesis.

AB - Background. In a previous analysis of head and neck squamous cell carcinomas (HNSCCs), we showed that the levels of the interferon-inducible protein IFI16 inversely correlate with cancer grade. In this study, we further evaluate the molecular role of IFI16 in the development of HNSCCs. Methods. The effect of IFI16 expression was evaluated by its retroviral restoration in an IFI16-negative HNSCC-derived cell line, HNO136. Growth rate and soft agar colony formation were evaluated. The effect of IFI16 restoration in cells exposed to doxorubicin was also analyzed. Results. IFI16 restoration resulted in the inhibition of both cell growth and in vitro transforming activity and increased doxorubicin-induced cell death by accumulating the cells at the G2/M phase. Conclusion. In agreement with our previous in vivo data, IFI16 appears to be involved in maintaining the normal growth of epithelial cells, whereas its downregulation may contribute to uncontrolled cell proliferation and tumorigenesis.

KW - Doxorubicin

KW - Growth arrest

KW - Head and neck squamous cell carcinoma

KW - IFI16

KW - Interferon

UR - http://www.scopus.com/inward/record.url?scp=37349076198&partnerID=8YFLogxK

U2 - 10.1002/hed.20611

DO - 10.1002/hed.20611

M3 - Article

SN - 1043-3074

VL - 29

SP - 835

EP - 844

JO - Head and Neck

JF - Head and Neck

IS - 9

ER -

Effects of IFI16 overexpression on the growth and doxorubicin sensitivity of head and neck squamous cell carcinoma-derived cell lines

Abstract

OSS delle Nazioni Unite

Accesso al documento

Altri file e collegamenti

Fingerprint

Cita questo