TY - JOUR
T1 - Effects of heavy metals on phospholipase C in gill and digestive gland of the marine mussel Mytilus galloprovincialis Lam
AU - Panfoli, I.
AU - Burlando, Bruno
AU - Viarengo, A.
PY - 2000
Y1 - 2000
N2 - We studied the in vivo and in vitro effects of Hg2+ and Cu2+ on the activity of phospholipase C (PLC), specific for phosphatidylinositol 4,5-bisphosphate, in the mussel (Mytilus galloprovincialis Lam). The enzyme activity was assayed in tissue homogenates from gills and digestive gland. The toxic effect of Hg2+ appeared to be stronger than that of Cu2+ both in vitro and in vivo, especially for the digestive gland. In in vitro tests, Hg2+ was able to inhibit PLC activity when added directly to the reaction mixture. Conversely, Cu2+ was effective only after preincubation, suggesting that the effect of the metal may be derived from lipid peroxidation due to Cu2+-induced oxyradical production. Treatment of mussels with sublethal concentrations of Hg2+ or Cu2+ in vivo produced significant PLC inhibition after 1 or 4 days, respectively. A recovery was reached after 7 days of in vivo metal incubation. Data indicate that in mussel gills and digestive gland heavy metals impair PLC activity, thereby affecting IP3-dependent Ca2+ signaling. (C) 2000 Elsevier Science Inc.
AB - We studied the in vivo and in vitro effects of Hg2+ and Cu2+ on the activity of phospholipase C (PLC), specific for phosphatidylinositol 4,5-bisphosphate, in the mussel (Mytilus galloprovincialis Lam). The enzyme activity was assayed in tissue homogenates from gills and digestive gland. The toxic effect of Hg2+ appeared to be stronger than that of Cu2+ both in vitro and in vivo, especially for the digestive gland. In in vitro tests, Hg2+ was able to inhibit PLC activity when added directly to the reaction mixture. Conversely, Cu2+ was effective only after preincubation, suggesting that the effect of the metal may be derived from lipid peroxidation due to Cu2+-induced oxyradical production. Treatment of mussels with sublethal concentrations of Hg2+ or Cu2+ in vivo produced significant PLC inhibition after 1 or 4 days, respectively. A recovery was reached after 7 days of in vivo metal incubation. Data indicate that in mussel gills and digestive gland heavy metals impair PLC activity, thereby affecting IP3-dependent Ca2+ signaling. (C) 2000 Elsevier Science Inc.
KW - Ca-signaling
KW - Heavy metals
KW - Marine invertebrates
KW - Mussels
KW - Phosphoinositides
KW - Phospholipase C
UR - http://www.scopus.com/inward/record.url?scp=0033743963&partnerID=8YFLogxK
U2 - 10.1016/S0305-0491(00)00272-8
DO - 10.1016/S0305-0491(00)00272-8
M3 - Article
SN - 0305-0491
VL - 127
SP - 391
EP - 397
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
IS - 3
ER -