Effects of heavy metals on phospholipase C in gill and digestive gland of the marine mussel Mytilus galloprovincialis Lam

I. Panfoli, Bruno Burlando, A. Viarengo

Risultato della ricerca: Contributo su rivistaArticolo in rivistapeer review

Abstract

We studied the in vivo and in vitro effects of Hg2+ and Cu2+ on the activity of phospholipase C (PLC), specific for phosphatidylinositol 4,5-bisphosphate, in the mussel (Mytilus galloprovincialis Lam). The enzyme activity was assayed in tissue homogenates from gills and digestive gland. The toxic effect of Hg2+ appeared to be stronger than that of Cu2+ both in vitro and in vivo, especially for the digestive gland. In in vitro tests, Hg2+ was able to inhibit PLC activity when added directly to the reaction mixture. Conversely, Cu2+ was effective only after preincubation, suggesting that the effect of the metal may be derived from lipid peroxidation due to Cu2+-induced oxyradical production. Treatment of mussels with sublethal concentrations of Hg2+ or Cu2+ in vivo produced significant PLC inhibition after 1 or 4 days, respectively. A recovery was reached after 7 days of in vivo metal incubation. Data indicate that in mussel gills and digestive gland heavy metals impair PLC activity, thereby affecting IP3-dependent Ca2+ signaling. (C) 2000 Elsevier Science Inc.

Lingua originaleInglese
pagine (da-a)391-397
Numero di pagine7
RivistaComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume127
Numero di pubblicazione3
DOI
Stato di pubblicazionePubblicato - 2000
Pubblicato esternamente

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