TY - JOUR
T1 - Effect of organic cosolvents on the stability and activity of the β- 1,4-galactosyltransferase from bovine colostrum
AU - Riva, Sergio
AU - Sennino, Barbara
AU - Zambianchi, Francesca
AU - Danieli, Bruno
AU - Panza, Luigi
PY - 1997/12
Y1 - 1997/12
N2 - The influence of various organic cosolvents on the stability and activity of the β-1,4-galactosyltransferase from bovine colostrum (GalT) and of its ancillary enzyme UDP-galactose-4'-epimerase has been investigated using the glucosylated alkaloid colchicoside (1) as a model substrate. It has been found that some cosolvents, such as Me2SO and MeOH, can be used up to 20% v/v without any influence on the performance of these enzymes, while others, such as tetrahydrofuran, rapidly inactivated GaIT at concentrations as low as 5% v/v. These results have been exploited for the galactosylation of the poorly water soluble coumarinic glucoside fraxin (2).
AB - The influence of various organic cosolvents on the stability and activity of the β-1,4-galactosyltransferase from bovine colostrum (GalT) and of its ancillary enzyme UDP-galactose-4'-epimerase has been investigated using the glucosylated alkaloid colchicoside (1) as a model substrate. It has been found that some cosolvents, such as Me2SO and MeOH, can be used up to 20% v/v without any influence on the performance of these enzymes, while others, such as tetrahydrofuran, rapidly inactivated GaIT at concentrations as low as 5% v/v. These results have been exploited for the galactosylation of the poorly water soluble coumarinic glucoside fraxin (2).
KW - Colchicoside
KW - Fraxin
KW - Organic cosolvents
KW - UDP-galactose-4'-epimerase
KW - β-1,4-Galactosyltransferase
UR - https://www.scopus.com/pages/publications/0031398924
U2 - 10.1016/S0008-6215(97)00273-5
DO - 10.1016/S0008-6215(97)00273-5
M3 - Article
SN - 0008-6215
VL - 305
SP - 525
EP - 531
JO - Carbohydrate Research
JF - Carbohydrate Research
IS - 3-4
ER -