TY - JOUR
T1 - Effect of different growth factors on human osteoblasts activities
T2 - A possible application in bone regeneration for tissue engineering
AU - Bosetti, Michela
AU - Boccafoschi, Francesca
AU - Leigheb, Massimiliano
AU - Cannas, Mario F.
N1 - Funding Information:
The research work is supported by University of Eastern Piedmont funds and by Piedmont Region funds 2005. The authors thank Prof. F. Renò, from the Human Anatomy Laboratory, Department of Experimental and Clinical Medicine, University of Eastern Piedmont “A. Avogadro” for the linguistic revision of the paper.
PY - 2007/12
Y1 - 2007/12
N2 - Cultured human primary osteoblasts reproduce the phenotypic differentiation and maturation of cells in vivo. We have investigated the influence of three isoforms of transforming growth factor beta (TGF-β1, TGF-β2 and TGF-β3), three fibroblast growth factors (FGF-2, FGF-4 and FGF-6) and the active metabolite of Vitamin D [1,25-(OH)2D3] on proliferation, alkaline phosphatase activity and mineralization of human osteoblasts during a period of 24 days of culture. TGF-β isoforms and three FGFs examined have been proved to be inducers of osteoblasts proliferation (higher extent for TGF-β and FGF-2) and inhibitors of alkaline phosphatase activity and osteoblasts mineralization. Combination of these growth factors with the active form of Vitamin D induced osteodifferentiation. In fact Vitamin D showed an additive effect on alkaline phosphatase activity and calcium content, induced by FGF-2 and TGF-β in human osteoblast. These results highlight the potential of proliferating cytokines' combination with mineralizing agents for in vitro bone growth induction in bone tissue engineering.
AB - Cultured human primary osteoblasts reproduce the phenotypic differentiation and maturation of cells in vivo. We have investigated the influence of three isoforms of transforming growth factor beta (TGF-β1, TGF-β2 and TGF-β3), three fibroblast growth factors (FGF-2, FGF-4 and FGF-6) and the active metabolite of Vitamin D [1,25-(OH)2D3] on proliferation, alkaline phosphatase activity and mineralization of human osteoblasts during a period of 24 days of culture. TGF-β isoforms and three FGFs examined have been proved to be inducers of osteoblasts proliferation (higher extent for TGF-β and FGF-2) and inhibitors of alkaline phosphatase activity and osteoblasts mineralization. Combination of these growth factors with the active form of Vitamin D induced osteodifferentiation. In fact Vitamin D showed an additive effect on alkaline phosphatase activity and calcium content, induced by FGF-2 and TGF-β in human osteoblast. These results highlight the potential of proliferating cytokines' combination with mineralizing agents for in vitro bone growth induction in bone tissue engineering.
KW - Active Vitamin D
KW - Alkaline phosphatase
KW - Growth factors
KW - Human primary osteoblast
KW - Mineralization
KW - Proliferation
UR - http://www.scopus.com/inward/record.url?scp=36248934276&partnerID=8YFLogxK
U2 - 10.1016/j.bioeng.2007.08.019
DO - 10.1016/j.bioeng.2007.08.019
M3 - Article
SN - 1389-0344
VL - 24
SP - 613
EP - 618
JO - Biomolecular Engineering
JF - Biomolecular Engineering
IS - 6
ER -