TY - JOUR
T1 - Ectomycorrhizal symbiosis affects functional diversity of rhizosphere fluorescent pseudomonads
AU - Frey-Klett, Pascale
AU - Chavatte, Michaël
AU - Clausse, Marie Lise
AU - Courrier, Sébastien
AU - Le Roux, Christine
AU - Raaijmakers, Jos
AU - Martinotti, Maria Giovanna
AU - Pierrat, Jean Claude
AU - Garbaye, Jean
PY - 2005/1
Y1 - 2005/1
N2 - Here we characterized the effect of the ectomycorrhizal symbiosis on the genotypic and functional diversity of soil Pseudomonas fluorescens populations and analysed its possible consequences in terms of plant nutrition, development and health. Sixty strains of P. fluorescens were isolated from the bulk soil of a forest nursery, the ectomycorrhizosphere and the ectomycorrhizas of the Douglas fir (Pseudostuga menziesii) seedlings-Laccaria bicolor S238N. They were characterized in vitro with the following criteria: ARDRA, phosphate solubilization, siderophore, HCN and AIA production, genes of N 2-fixation and antibiotic synthesis, in vitro confrontation with a range of phytopathogenic and ectomycorrhizal fungi, effect on the Douglas fir-L., bicolor symbiosis. For most of these criteria, we demonstrated that the ectomycorrhizosphere significantly structures the P. fluorescens populations and selects strains potentially beneficial to the symbiosis and to the plant. This prompts us to propose the ectomycorrhizal symbiosis as a true microbial complex where multitrophic interactions take place. Moreover it underlines the fact that this symbiosis has an indirect positive effect on plant growth, via its selective pressure on bacterial communities, in addition to its known direct positive effect.
AB - Here we characterized the effect of the ectomycorrhizal symbiosis on the genotypic and functional diversity of soil Pseudomonas fluorescens populations and analysed its possible consequences in terms of plant nutrition, development and health. Sixty strains of P. fluorescens were isolated from the bulk soil of a forest nursery, the ectomycorrhizosphere and the ectomycorrhizas of the Douglas fir (Pseudostuga menziesii) seedlings-Laccaria bicolor S238N. They were characterized in vitro with the following criteria: ARDRA, phosphate solubilization, siderophore, HCN and AIA production, genes of N 2-fixation and antibiotic synthesis, in vitro confrontation with a range of phytopathogenic and ectomycorrhizal fungi, effect on the Douglas fir-L., bicolor symbiosis. For most of these criteria, we demonstrated that the ectomycorrhizosphere significantly structures the P. fluorescens populations and selects strains potentially beneficial to the symbiosis and to the plant. This prompts us to propose the ectomycorrhizal symbiosis as a true microbial complex where multitrophic interactions take place. Moreover it underlines the fact that this symbiosis has an indirect positive effect on plant growth, via its selective pressure on bacterial communities, in addition to its known direct positive effect.
KW - Bacteria
KW - Ectomycorrhizosphere effect
KW - Functional diversity
KW - Laccaria bicolor S238N
KW - Pseudomonas fluorescens
KW - Pseudotsuga menziesii
UR - http://www.scopus.com/inward/record.url?scp=11144260081&partnerID=8YFLogxK
U2 - 10.1111/j.1469-8137.2004.01212.x
DO - 10.1111/j.1469-8137.2004.01212.x
M3 - Article
SN - 0028-646X
VL - 165
SP - 317
EP - 328
JO - New Phytologist
JF - New Phytologist
IS - 1
ER -