Drug sensitivity tests after cell separation on density gradients

L. Silvestro; I. Viano; G. Compagnoni; M. L. Soffiantino; C. Vercellone; E. Genazzani

Drug sensitivity tests after cell separation on density gradients

L. Silvestro
, I. Viano
, G. Compagnoni
, M. L. Soffiantino
, C. Vercellone
, E. Genazzani

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

Cells from two leukaemic cell lines (K562 and L1210) were separated on a discontinuous Percoll gradient with densities from 1040 to 1090 by centrifugation. The cells recovered from gradient interfaces were checked for their DNA content and subjected to two different drug sensitivity tests using three cytostatic drugs. The purpose of the study was to compare the sensitivity of a sample of the whole population with that of the fractions thus obtained. Depending on the test used and the line tested, there was a 5.5 to 55.5% discordance between the results for the fractions and the non-separated cells.

Lingua originale	Inglese
pagine (da-a)	119-123
Numero di pagine	5
Rivista	Anticancer Research
Volume	8
Numero di pubblicazione	1
Stato di pubblicazione	Pubblicato - 1988
Pubblicato esternamente	Sì

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title = "Drug sensitivity tests after cell separation on density gradients",

abstract = "Cells from two leukaemic cell lines (K562 and L1210) were separated on a discontinuous Percoll gradient with densities from 1040 to 1090 by centrifugation. The cells recovered from gradient interfaces were checked for their DNA content and subjected to two different drug sensitivity tests using three cytostatic drugs. The purpose of the study was to compare the sensitivity of a sample of the whole population with that of the fractions thus obtained. Depending on the test used and the line tested, there was a 5.5 to 55.5\% discordance between the results for the fractions and the non-separated cells.",

author = "L. Silvestro and I. Viano and G. Compagnoni and Soffiantino, \{M. L.\} and C. Vercellone and E. Genazzani",

year = "1988",

language = "English",

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T1 - Drug sensitivity tests after cell separation on density gradients

AU - Silvestro, L.

AU - Viano, I.

AU - Compagnoni, G.

AU - Soffiantino, M. L.

AU - Vercellone, C.

AU - Genazzani, E.

PY - 1988

Y1 - 1988

N2 - Cells from two leukaemic cell lines (K562 and L1210) were separated on a discontinuous Percoll gradient with densities from 1040 to 1090 by centrifugation. The cells recovered from gradient interfaces were checked for their DNA content and subjected to two different drug sensitivity tests using three cytostatic drugs. The purpose of the study was to compare the sensitivity of a sample of the whole population with that of the fractions thus obtained. Depending on the test used and the line tested, there was a 5.5 to 55.5% discordance between the results for the fractions and the non-separated cells.

AB - Cells from two leukaemic cell lines (K562 and L1210) were separated on a discontinuous Percoll gradient with densities from 1040 to 1090 by centrifugation. The cells recovered from gradient interfaces were checked for their DNA content and subjected to two different drug sensitivity tests using three cytostatic drugs. The purpose of the study was to compare the sensitivity of a sample of the whole population with that of the fractions thus obtained. Depending on the test used and the line tested, there was a 5.5 to 55.5% discordance between the results for the fractions and the non-separated cells.

UR - https://www.scopus.com/pages/publications/0023940747

M3 - Article

SN - 0250-7005

VL - 8

SP - 119

EP - 123

JO - Anticancer Research

JF - Anticancer Research

IS - 1

ER -

Drug sensitivity tests after cell separation on density gradients

Abstract

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