TY - JOUR
T1 - DNA typing from epiglottic cartilage of exhumed bodies
AU - Gino, S.
AU - Robino, C.
AU - Bonanno, E.
AU - Torre, C.
N1 - Funding Information:
This work was supported by the Director, Office of Science, Office of Basic Energy Sciences, Materials Sciences Division, of the U.S. Department of Energy under Contract DE-AC03-76SF00098. The authors also thank P. J. Grandinetti and co-workers for providing a preprint of their FASTER MQMAS work.
PY - 2003/1/1
Y1 - 2003/1/1
N2 - Epiglottic cartilage was evaluated as an alternative source of genomic DNA from decomposed human remains to be used in forensic casework. The histological study of epiglottic cartilage samples, taken from three bodies exhumed after 6–18 months, showed the presence of a well-conserved matrix delimiting the lacunae, where residual chondrocytes could be seen. The amount of genomic DNA isolated from the samples by means of a standard organic phenol/chloroform method varied from 0.25 to 2 ng/mg of tissue. Coamplification of six short tandem repeat (STR) loci plus Amelogenin, followed by capillary electrophoresis, generated full profiles for all the DNA extracts. In one case, it was possible to compare the obtained profile with that from a reference blood sample collected at the time of postmortem examination: Complete concordance of genotypes was observed at all loci.
AB - Epiglottic cartilage was evaluated as an alternative source of genomic DNA from decomposed human remains to be used in forensic casework. The histological study of epiglottic cartilage samples, taken from three bodies exhumed after 6–18 months, showed the presence of a well-conserved matrix delimiting the lacunae, where residual chondrocytes could be seen. The amount of genomic DNA isolated from the samples by means of a standard organic phenol/chloroform method varied from 0.25 to 2 ng/mg of tissue. Coamplification of six short tandem repeat (STR) loci plus Amelogenin, followed by capillary electrophoresis, generated full profiles for all the DNA extracts. In one case, it was possible to compare the obtained profile with that from a reference blood sample collected at the time of postmortem examination: Complete concordance of genotypes was observed at all loci.
KW - DNA typing
KW - Forensic medicine
KW - Human remains
KW - Short tandem repeats
UR - http://www.scopus.com/inward/record.url?scp=85023077335&partnerID=8YFLogxK
U2 - 10.1016/S0531-5131(02)00341-2
DO - 10.1016/S0531-5131(02)00341-2
M3 - Article
AN - SCOPUS:85023077335
SN - 0531-5131
VL - 1239
SP - 885
EP - 887
JO - International Congress Series
JF - International Congress Series
IS - C
ER -
