TY - JOUR
T1 - DNA prime and protein boost immunization with innovative polymeric cationic core-shell nanoparticles elicits broad immune responses and strongly enhance cellular responses of HIV-1 tat DNA vaccination
AU - Castaldello, Arianna
AU - Brocca-Cofano, Egidio
AU - Voltan, Rebecca
AU - Triulzi, Chiara
AU - Altavilla, Giuseppe
AU - Laus, Michele
AU - Sparnacci, Katia
AU - Ballestri, Marco
AU - Tondelli, Luisa
AU - Fortini, Cinzia
AU - Gavioli, Riccardo
AU - Ensoli, Barbara
AU - Caputo, Antonella
N1 - Funding Information:
This work was supported by grants from the Italian Concerted Action on HIV-AIDS Vaccine Development (ICAV) and from MURST 40%. We are grateful to M. Magnani (Diatheva, Fano, Italy) for providing the Tat protein, to R. De Michele and M. Scarletti (University of Ferrara) for excellent technical work and animal care, and to R. Guerrini (UFPeptide, s.r.l., Ferrara) for synthesis of the Tat peptide.
PY - 2006/7/17
Y1 - 2006/7/17
N2 - Novel biocompatible core-shell cationic nanoparticles, composed of an inner hard core of poly(methylmethacrylate) (PMMA) and a hydrophilic tentacular shell bearing positively charged groups and poly(ethyleneglycol) chains covalently bound to the core, were prepared by emulsion polymerization and characterized in vitro and in vivo for DNA vaccine applications. The nanoparticles reversibly adsorbed large amounts of DNA, mainly through electrostatic interactions, preserved its functional structure, efficiently delivered it intracellularly, and were not toxic in vitro or in mice. Furthermore, two intramuscular (i.m.) immunizations (4 weeks apart) with a very low dose (1 μg) of the plasmid pCV-tat delivered by these nanoparticles followed by one or two protein boosts induced significant antigen-specific humoral and cellular responses and greatly increased Th1-type T cell responses and CTLs against HIV-1 Tat.
AB - Novel biocompatible core-shell cationic nanoparticles, composed of an inner hard core of poly(methylmethacrylate) (PMMA) and a hydrophilic tentacular shell bearing positively charged groups and poly(ethyleneglycol) chains covalently bound to the core, were prepared by emulsion polymerization and characterized in vitro and in vivo for DNA vaccine applications. The nanoparticles reversibly adsorbed large amounts of DNA, mainly through electrostatic interactions, preserved its functional structure, efficiently delivered it intracellularly, and were not toxic in vitro or in mice. Furthermore, two intramuscular (i.m.) immunizations (4 weeks apart) with a very low dose (1 μg) of the plasmid pCV-tat delivered by these nanoparticles followed by one or two protein boosts induced significant antigen-specific humoral and cellular responses and greatly increased Th1-type T cell responses and CTLs against HIV-1 Tat.
KW - Biocompatible nanoparticles
KW - Cellular responses
KW - DNA vaccination
KW - HIV-1 tat
UR - https://www.scopus.com/pages/publications/33745284588
U2 - 10.1016/j.vaccine.2006.05.058
DO - 10.1016/j.vaccine.2006.05.058
M3 - Article
SN - 0264-410X
VL - 24
SP - 5655
EP - 5669
JO - Vaccine
JF - Vaccine
IS - 29-30
ER -
