Development and validation of an ELISA method for detection of growth arrest specific 6 (GAS6) protein in human plasma

F ALCIATO; Pier Paolo SAINAGHI; Luigi Mario CASTELLO; L BERGAMASCO; S CARNIELETTO; Gian Carlo AVANZI

doi:10.1080/15321810801888480

Development and validation of an ELISA method for detection of growth arrest specific 6 (GAS6) protein in human plasma

F ALCIATO, Pier Paolo SAINAGHI, Luigi Mario CASTELLO, L BERGAMASCO, S CARNIELETTO, Gian Carlo AVANZI

Dipartimento di Medicina Traslazionale

Risultato della ricerca: Contributo su rivista › Articolo in rivista › peer review

Abstract

Gas6 protein is possibly involved in human diseases, but a validated plasma assay is lacking. So, we developed a sandwich enzyme-linked immunosorbent assay (ELISA) method using commercially available reagents. An appropriate plasma-based matrix was prepared to optimize the assay. The ELISA method showed inter- and intra-assay coefficients of variation lower than 15%. Recoveries all fell within 15% of expected values. Plasma Gas6 concentration in 61 healthy donors was 20.3+/-3.8 ng/mL. Our assay meets FDA requirements for precision and accuracy for the validation of bioanalytical methods and it is suitable for research or diagnostic purposes.

Lingua originale	Inglese
pagine (da-a)	167-180
Numero di pagine	14
Rivista	Journal of Immunoassay and Immunochemistry
Volume	29
Numero di pubblicazione	2
DOI	https://doi.org/10.1080/15321810801888480
Stato di pubblicazione	Pubblicato - 2008

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abstract = "Gas6 protein is possibly involved in human diseases, but a validated plasma assay is lacking. So, we developed a sandwich enzyme-linked immunosorbent assay (ELISA) method using commercially available reagents. An appropriate plasma-based matrix was prepared to optimize the assay. The ELISA method showed inter- and intra-assay coefficients of variation lower than 15\%. Recoveries all fell within 15\% of expected values. Plasma Gas6 concentration in 61 healthy donors was 20.3+/-3.8 ng/mL. Our assay meets FDA requirements for precision and accuracy for the validation of bioanalytical methods and it is suitable for research or diagnostic purposes.",

author = "F ALCIATO and SAINAGHI, \{Pier Paolo\} and CASTELLO, \{Luigi Mario\} and L BERGAMASCO and S CARNIELETTO and AVANZI, \{Gian Carlo\}",

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AU - ALCIATO, F

AU - SAINAGHI, Pier Paolo

AU - CASTELLO, Luigi Mario

AU - BERGAMASCO, L

AU - CARNIELETTO, S

AU - AVANZI, Gian Carlo

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AB - Gas6 protein is possibly involved in human diseases, but a validated plasma assay is lacking. So, we developed a sandwich enzyme-linked immunosorbent assay (ELISA) method using commercially available reagents. An appropriate plasma-based matrix was prepared to optimize the assay. The ELISA method showed inter- and intra-assay coefficients of variation lower than 15%. Recoveries all fell within 15% of expected values. Plasma Gas6 concentration in 61 healthy donors was 20.3+/-3.8 ng/mL. Our assay meets FDA requirements for precision and accuracy for the validation of bioanalytical methods and it is suitable for research or diagnostic purposes.

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JF - Journal of Immunoassay and Immunochemistry

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ER -

Development and validation of an ELISA method for detection of growth arrest specific 6 (GAS6) protein in human plasma

Abstract

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