TY - JOUR
T1 - Determination of H2S solubility via the reaction with ferric hemoglobin I from the bivalve mollusc Lucina pectinata
AU - Boffi, Alberto
AU - Rizzi, Menico
AU - Monacelli, Fabrizio
AU - Ascenzi, Paolo
N1 - Funding Information:
The authors wish to thank Prof. M. Bolognesi and Prof. J.B. Wittenberg for helpful discussions. This work was supported by grants from the Ministry of University, Scientific Research and Technology of Italy (target oriented project ‘Biocatalisi e Bioconversioni’) and from the National Research Council of Italy (target oriented project ‘Biotecnologie’).
PY - 2000/10/18
Y1 - 2000/10/18
N2 - A new, simple and fast spectrophotometric method for the determination of the H2S concentration is reported. This method, based on the 1:1 reaction between H2S and the ferric derivative of hemoglobin I (HbI) from the bivalve mollusc Lucina pectinata, allows the quantitative determination of H2S dissolved in a given solution even at concentrations as low as 1 x 10-6 M. Note that L. pectinata HbI is considered the physiological receptor of H2S. (C) 2000 Elsevier Science B.V.
AB - A new, simple and fast spectrophotometric method for the determination of the H2S concentration is reported. This method, based on the 1:1 reaction between H2S and the ferric derivative of hemoglobin I (HbI) from the bivalve mollusc Lucina pectinata, allows the quantitative determination of H2S dissolved in a given solution even at concentrations as low as 1 x 10-6 M. Note that L. pectinata HbI is considered the physiological receptor of H2S. (C) 2000 Elsevier Science B.V.
KW - HS binding to Lucina pectinata hemoglobin I
KW - HS solubility
KW - Lucina pectinata hemoglobin I
UR - http://www.scopus.com/inward/record.url?scp=0034684222&partnerID=8YFLogxK
U2 - 10.1016/S0304-4165(00)00123-9
DO - 10.1016/S0304-4165(00)00123-9
M3 - Article
SN - 0304-4165
VL - 1523
SP - 206
EP - 208
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
IS - 2-3
ER -